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Apoptosis of Human Osteosarcoma MG-63 Cells Induced by Dihydroartemisinin
Ma Yufan1, Lu Tingli1, Wang Yunqing1, Zhao Wen1, Mei Qibing1, Chen Tao1,2,3∗
1Key Laboratory for Space Bioscience and Biotechnology, Faculty of Life Science, Northwestern Polytechnical University, Xi’an 710072,China; 2Shaanxi Liposome Research Center, Xi’an 710075, China; 3Xi’an Libang Pharmaceutic
Abstract: The apoptosis effects of dihydroartemisinin (DHA) on human osteosarcoma MG-63 cells have been studied using the methods of CCK-8 staining， flow cytometry analysis， Hoechst33258 staining， Annexin VFITC staining and electron microscope. The results showed that DHA decreased the cell viability in a concentration and time-dependent manner. The IC50 values of DHA against the MG-63 cells were determined as 44.87 μmol/L and 19.15 μmol/L after 48 h and 72 h， respectively. The results of flow cytometry analysis showed that DHA could induce the emergence of the phase of apoptosis， the rate is 45.10% and the cell cycle is blocked in G0/G1 phase. Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining. This study suggested that DHA had induced apoptosis of the human osteosarcoma MG-63 cells effectively， and provided foundation and research proofs to study more about the apoptosis mechanisms of the osteosarcoma cells.
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