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The Effect of Plasticizer DEHP on Invasion and Migration of Human Extravillous Trophoblast Cells
Li Rui1, Yu Chao2, Ding Yubin1, Chen Xuemei1, Liu Xueqing1, Huang Pei1, Wang Yingxiong1,2, He Junlin1*
1Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing 400016, China;2Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
Abstract: This article aims to investigate the effect and mechanism of di-(2-ethylhcxyl) phthalate (DEHP) on invasion and migration of human extravillous trophoblast HTR-8/Svneo cells. We used MTT assay and flow cytometry to determine the optimum concentration of DEHP treating to HTR-8/Svneo cells. Cell invasion and migration were measured by Transwell chambers. The transcription level of mRNA and expression level of protein which related to cell invasion and migration were detected by Real-time PCR and Western blot. The results showed that DEHP enhanced HTR-8/Svneo cells invasion and migration as well as up-regulated expression level of MMP-2 and MMP-9. We also determined that DEHP increased the phosphorylated of p38 and JNK protein. In addition， p38 and JNK inhibitors treated with HTR-8/Svneo cells resulted in decreased cell invasion which can be increased by DEHP. In conclusion， our findings suggested that DEHP can enhance the invasion and migration of HTR-8/Svneo cells， which is， at least， partly regulated by phosphorylated p38 and JNK activation and relevant with MMP-2 and MMP-9 expression.
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