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Effect of Caveolin-1 on Proliferation and Survival of MCF-7 Breast Cancer Cells
Lu Ruisheng1, Ma Ji1, Zhang Yan1,2, Yu Lingli1,2, Ambre Spencer2, David Cluet2,Brian B. Rudkin1,2*, Yuan Chonggang1*
1School of Life Sciences, EastChinaNormal Univercity, Shanghai 200062, China; 2Differentiation & Cell Cycle Group, Cellular Molecular Biology Laboratory, UMR 5239 CNRS/ENS Lyon/University of Lyon 1, IFR 128, Bioscience Lyon-Gerland.
Abstract: We Study the effect of Caveolin-1 on the proliferation and survival in MCF-7 breast cancer cells. A very low expression level was detected by Western blot in 6 breast cancer cell lines. Caveolin-1 expression increased dramatically after transient transfection and has its biological activity. The MTT assay showed that the proliferation rate of MCF-7 cells was decreased after the transfection of Caveolin-1. The cell apoptosis relative protein was observed by Western blot and immunofluorescence. The quantity of phosphorylated P38 and the expression of Bax increased. We concluded that Caveolin-1 expression inhibited cells proliferation and survival and induces the Bax mediated cell apoptosis in MCF-7 breast cancer cells.
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