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Localization of ezrin Enhancer in Nasopharyngeal Carcinoma Cells
Gao Shuying, Dai Yanpeng, Yin Meijun, Long Xia, Yu Jie*
Central Laboratory, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China
Abstract: To localize human ezrin gene enhancer in nasopharyngeal carcinoma cells， a series of reporter gene expression vectors were constructed using nested-deletion method and then were detected via luciferase assay. In CNE2 cells， it was found that ezrin –1541/–706 possessed promoter and enhancer activity， and there were some positive and negative transcriptional regulatory regions within this –1541/–706 region. Further studies on 5 potential transcriptional regulatory regions revealed that ezrin –1297/–1186 enhanced transcription of ezrin promoter and SV40 promoter significantly， other 4 regions showed absent regulation or weaker enhancement on promoters. These data suggested that the ezrin –1297/–1186， a key transcriptional regulatory region as an enhancer， probably regulated ezrin transcription in company with other potential regulatory regions.
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