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Recombinant Human S100A6 Promotes the Proliferation， Migration and Invasion but Inhibits the Apoptosis of Human Breast Cancer Cell Line MCF-7
You Li, Xu Lanlan, Guo Yuanyuan, Sun Shuangshuang, Zou Zhengyu, Li Yuye,Luo Jinyong, He Tongchuan, Zhou Lan*
Key Laboratory of Laboratory Medical Diagnostics, Department of Medical Laboratory, Ministry of Education,Chongqing Medical University, Chongqing 400016, China
Abstract: To aim at effects of human S100A6 on proliferation， apoptosis， migration and invasion of human breast cancer cell line MCF-7， recombinant protein GST-hS100A6 was purified from bacteria BL21 that identified as 36 kDa by SDS-PAGE and recognized by S100A6 antibody with Western blot. MCF-7 were treated by GSThS100A6 with different concentrations while GST as a control group. MTT was used to detect the cell proliferation. Results showed that after 48 h， the D492 value of 100 μg/mL and 300 μg/mL group of GST-hS100A6 increased by 29.1% and 84.6% compared with GST group， respectively (P<0.05). Colony-forming assay showed that the ability of colony formation of GST-hS100A6 increased by 38.7% compared with GST group (P<0.05). Hoechst staining showed that cell apoptosis rate of GST-hS100A6 group decreased by 67.8% compared with GST group at 24 h (P<0.05) and decreased by 58.4% at 48 h (P<0.05). Results of wound healing assay indicated that the healing rate of GST-hS100A6 group was 2.2 times of GST group (P<0.05) at 24 h. Transwell invasion assay showed that the transmembrane cell number of GST-hS100A6 group increased by 88.1% (P<0.05) compared with GST group at 24 h. To sum up， S100A6 could promote cell proliferation， colony formation， migration， and invasion， but inhibit cell apoptosis on human breast cancer cell line MCF-7， indicates that S100A6 has a promoting effect on human breast cancer which would be a new molecular target for treatment of human breast cancer.
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