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The Experimental Study on Cytotoxic Effect of Oncolytic Virus ZD55-TRAIL Combined With Rapamycin against Human Cancer Cells and the Exploration Mechanism
Xi-Jun Liu1, Xin Li1, Shui-Di Zheng1, Dan Zhong1, Kang-Jian Zhang2, Shi-Bing Wang1, Xiu-Mei Zhou1*
1Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;2Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Abstract: This research investigates the effect of combined application of oncolytic virus ZD55-TRAIL and the chemotherapeutic drugs Rapamycin (Rap) in growth inhibiton of human cancer cells in vitro. The effects of the treatment were measured by MTT assay and the replication of E1B-55KD deletion adenovirus was detected by the expression of E1A protein by Western blot. These observations suggest that Rapamycin could increase the levels of E1A， which may explain its ablitiy to enhance ZD55-TRAIL virus oncolysis. The combination therapy with signaling inhibitors that modulate activity of mTOR pathway will promote the cytotoxity against human cancer cells.
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