Home > Browse Issues > Vol.33 No.6
Effects of TNF-α and IL-13 on Collagen Synthesizing of Human Lung Fibroblast
Hou-Wen Chen1, Meng-Zhou Guo2, Qi Chen3, Chao Wu2, Xiang-Long Li2,Jie Fan2, Zhi-Yong Xiong2, Meng-Bo Zhu3, Xiao-Yu Shi4*
1Department of Medicine, Graduate School of Nanchang University, Nanchang 330006, China; 2The First Clinical Medical School of Nanchang University, Nanchang 330006, China; 3The Second Clinical Medical School of Nanchang Un
Abstract: Interstitial fibrosis， characterized by excessive collagen accumulation， is a pathological hallmark of chronic pulmonary diseases. In this study， gene expression alterations of IL-13Rα1， IL-13Rα2 and collagen type one were monitored by RT-PCR in human lung fibroblasts response to the treatment of various dosages of TNF-α and IL-13， sIL-13Rα2 secreted in culture medium was quantitated by ELISA and collagen synthesized in cells was determined by hydroxyproline-assaying. We found that， IL-13Rα2， but not IL-13Rα1， was upregulated significantly in human lung fibroblasts at both mRNA and protein levels by the treatment of both TNF-α and IL-13. However， collagen synthesis in human lung fibroblasts was inhibited only by TNF-α treatment.
CN
EN