The Extracellular Regulated Kinase 1/2 is Involved in the Inhibition of Matrix Metalloproteinase-9 by Transforming Growth Factor-β1 in Trophoblast Cells

Abstract: Normal trophoblast invasion is stringently controlled by transforming growth factor-β (TGF-β). In this study， we investigated TGF-β1-mediated regulation of matrix metalloproteinase (MMP)-2， and -9 in human normal trophoblast cells (first trimester cytotrophoblast， CTB). The results showed that TGF-β1 inhibited MMP- 9 mRNA abundance and the level of pro-enzyme secretion in CTB cells， but did not affect MMP-2 expression. Interleukin-1β (IL-1β) and TGF-β1 both showed inhibitory but mutually antagonistic effects on MMP-9 mRNA and pro-enzyme expression. Furthermore， inhibition of the extracellular signal-regulated kinase 1/2 (ERK1/2) blocked TGF-β1-reduced MMP-9 mRNA and pro-MMP-9 expression. These observations suggest that normal trophoblast invasion is controlled by TGF-β1 through inhibiting MMP-9 activity via ERK1/2.

CSTR: 32200.14.cjcb.2011.06.0008