Home > Browse Issues > Vol.30 No.1
Cloning and Characterization of Three Actin Genes in Gynoecious Line Cucumber (Cucumis sativus L.)
Ji-Ming Xu, Tai-He Xiang*
College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China
Abstract: Three actin genes with length of 1 186 bp， 955 bp and 870 bp named as Act1， Act2 and Act3 (GenBank accession number: DQ115881， DQ115882 and DQ115883) from gynoecious cucumber line were cloned in one-step PCR reaction. The cloned genes share high similarity to the 2th exon， 3th exon and 2th intron of other actin gene in the GenBank (AF386514.1， AF059484.1 and AB010922.1 etc.). The analysis of RT-PCR revealed that Act1 expressed in root only， the Act2 could be detected in root and female flower， while the Act3 expressed in all tested organs. The cloned genes enriched the actin gene family and could be employed to study molecular and physiological mechanism of actin gene. In addition， the result indicates that the origin and evolution of plant actin genes were highly homologous and conservative respectively.
CN
EN