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Lipopolysacchride-induced Motor Neuron Injury in the Anterior Horn of Spinal Cord
Bin Li1, Hui Dong1,2, Zhe Li1, Hui Bu1,2, Xiao-Yun Liu1,2, Meng-Meng Sun1, Chun-Yan Li1,2*
1Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China; 2Cardiocerebrovascular Disease Institute of Hebei Province, Shijiazhuang 050000, China
Abstract: The present study was undertaken to investigate the mechanisms involved in lipopolysaccharide (LPS)-induced motor neuron injury. Organotypic spinal cord slice cultures were treated with medium alone or LPS with different concentrations. Neuron injury was measured using immunohistochemistry， enzyme activity assay and electromicroscopy. In addition， LPS group was treated with BAPTA-AM and apocynin respectively， and motor neuron survival was evaluated by SMI-32 immunohistochemistry. The results showed that LPS could induce dose- and time- dependent loss of motor neurons and elevation of LDH concentrations in the culture medium. The ultrastructure of motor neurons showed obvious changes. In contrast， interneurons in the dorsal horn were impaired slightly. Motor neuron lacked the expression of calretinin， and BAPTA-AM ameliorated motor neuron injury， indicating that the low capacity of Ca2+ buffering is one of the factors responsible for the vulnerability of motor neurons. NADPH oxidase was activated upon LPS challenge and apocynin had neuroprotective potential on LPS-induced motor neuron death， suggesting that NADPH oxidase may play an important role in inflammation-mediated motor neuron injury.
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