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Functional Analysis of MoCMKK2 Gene in Magnaporthe oryzae
Rong-Lin He, Rong-Hui Fan, Jian-Ping Lu1, Fu-Cheng Lin*, Xiao-Hong Liu
Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; 1College of Life Sciences, Zhejiang University, Hangzhou 310058, China
Abstract: Calicium is an important intracellular second messenger and the Ca2+-signal transduction pathway is involved nearly every aspects of the cellular signal transduction network. In this study， the function of a calcium/calmodulin-dependent protein kinase kinase 2 (MoCMKK2) gene in the development of the rice blast fungus Magnaporthe oryzae was studied with a targeted gene disruption method. The sporulation of the null mutant was dramatically increased; however， the pathogenicity， mycelial growth rates， mycelial growth under starvation， conidial germination， appressorium formation and mating test of the MoCMKK2 null mutant were comparable with those of the wild-type strain. These data suggest that the MoCMKK2 gene is a negative factor to regulate sporulation. These findings will further our understanding of the disease infection cycle of Magnaporthe oryzae.
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