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Effects of Quercetin on Growth of Colon Cancer Cell Line RKO
Sai-Nan Tan1,2, Cai-Ling Lu2, Yi Cui2, Xu Ma2*
1Graduate School of Peking Union Medical College, Beijing 100730, China; 2Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China
Abstract: MTT assay tested cell growth of colon cancer cell line RKO; cell cycle and cell apoptosis was observed by flow cytometry technique; RT-PCR and Western blot technique detected the expression of p21， p27 and p53. MTT assay showed that quercetin significantly inhibited cell growth and had dose-dependent effect; Flow cytometry technique showed that with 5 μmol/L quercetin the cell cycle was arrested in G0/G1 phase; apoptosis rates of 5， 10， 20 μmol/L groups were 23.4%， 24.2%， 47.9%， and apoptosis rate of the control group was 13.2%; mRNA and protein expression level of p21 and p27 increased， the expression level of pro-apoptotic protein p53 increased. Quercetin has significant growth inhibition on RKO cells. Cell cycle is arrested in G0/G1 phase by upregulating the expression of p21 and p27; quercetin may also induce apoptosis by upregulating the expression of p53.
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