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Monitoring Colitis Development in Mice by Micro-CT Colonography
Wei-Gang Wang1,2, Zhen-Ze Liu2, Hui-Min Yan3*
1Department of Chemistry, Tongji University, Shanghai 200092, China; 2School of Life Science and Technology, Tongji University,Shanghai 200092, China; 3Shanghai Research Center for Model Organisms, Shanghai 201203, China
Abstract: To evaluate colon wall thickness noninvasively in the dextran sodium sulfate (DSS) induced mouse model of colitis， we performed micro-CT colonography. Mice were scanned by micro-CT at day 0， day 8 and day 13 after induction of colitis and calculated the colon wall thickness. Iopamidol was used by oral and intraperitoneal injection to show the colon wall. The value of the descending colon wall thickness were 0.4586±0.04 mm (day 8) and 0.40325±0.03 mm (day 13) significantly (P<0.001) higher than the control group (0.28±0.02 mm). The related body weight loss， clinical score， histological section and score were consistent with the result of micro-CT. Thus， the results suggested that micro-CT can be used directly， consecutively and noninvasively in monitoring of the inflammatory response in mouse colitis in future studies.
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