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Inhibiting Effects and Its Mechanisms of Ginsenoside Rg1 on Human Gastric Cancer Cell Line in vitro
Yi Shang1,2, Cai-Quan Zhang1*
1Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2North Sichuan Medical College, Nanchong 637000, China
Abstract: To explore the inhibitory effects of ginsenoside Rg1 on the human gastric carcinoma cell line BGC-823 in vitro， 24， 48 and 72 h after treated with different concentration of GS-Rg1， the cell proliferation of the BGC-823 cells was valuated by MTT assay， cell cycle distribution was measured by flow cytometric analysis and the expression of p16INK4a and p21WAF1 genes mRNA were detected by reverse transcription PCR (RT-PCR). Results showed that with the increase of concentration of GS-Rg1 and the duration of administration， the inhibitory effects were elevated (P﹤0.05). The proportion of cells in G0/G1 phase was obviously increased whereas that in G2/S phase was decreased. The expression of p16INK4a and p21WAF1 genes mRNA were upregulated. These results suggested that GS-Rg1 can inhibit the proliferation of BGC-823 cells in vitro by upregulating the expression of p16INK4a and p21WAF1 genes mRNA.
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