Home > Browse Issues > Vol.33 No.3
Effects of Protein Kinase B/Akt on Polymerization of the Actin in Mouse Oocyte and Embryos by Laser Confocal Microscopy
Di-Di Wu, Ying-Hua Ju, Jun Meng, Chao Liu, Chen Fen, Bing-Zhi Yu*
Department of Biochemical and Molecular Biology, China Medical University, Shenyang 110001, China
Abstract: In the present study we aimed at elucidating the polymerization of the actin in mouse oocytes and embryos after treated with different kinase active mRNA of PKB. We observed the expression of actin and the polymerization after treated with PKB mRNA in the mouse oocytes and embryos by using modified immunofluorescent staining and laser confocal microscopy. The results showed that the actin was expressed in mouse oocytes and embryos mostly at spindle， polar body and contractile ring. Injection of mRNA coding for a constitytively active myristoylated PKB/Akt into oocytes or embryos induced polymerization of actin， whereas microinjection of mRNA of kinse-deficient PKB/Akt inhibited the formation of actin storage. So our findings confirmed that PKB activation was necessary for polymerization of actin in mouse oocytes and early embryos.
CN
EN