Influences of IFN-γ on the Proliferation and Apoptosis of Laryngeal Carcinoma Hep-2 Cells

Abstract: human laryngeal cell line Hep-2 cells were cultured and treated with 10 ng/ml IFN-γ for different time. After IFN-γ treatment， cell counting， cell apoptosis-DNA ladder analysis and semi-quantitative RTPCR methods were used to explore the influences of IFN-γ on the proliferation and apoptosis of Hep-2 cells and its preliminary molecular mechanism was analyzed simultaneously. The cell growth assay result showed that from the second day on Hep-2 cells treated with IFN-γ proliferated obviously slower than the untreated control cells. Cell apoptosis analysis showed that the genomic DNA of Hep-2 cells displayed as the DNA ladder pattern in gel electrophoresis when the Hep-2 cells were treated with IFN-γ. Semi-quantitative RT-PCR analysis showed that the expression of IFI16 gene was induced in Hep-2 cells after treated with IFN-γ. Based on the above results， it was concluded that IFN-γ could inhibit Hep-2 cells proliferation and induce Hep-2 cells apoptosis， and the mechanism for IFN-γ inhibiting Hep-2 cells proliferation and inducing Hep-2 cells apoptosis is probably related to the IFN-γ- induced IFI16 gene expression.

CSTR: 32200.14.cjcb.2011.03.0005