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The Expression of MBD2 and Related Gene Methylation Microarray in Hydatidiform Mole
Liang-Rui Guo, Jun-Lin He, Xue-Mei Chen, Xue-Qing Liu, Ying-Xiong Wang, Yu-Bin Ding*
Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: The expression of MBD2 (methyl-CpG-binding domain2) in complete hydatidiform mole (CHM) and normal early chorionic villi were examined by Real-time RT-PCR， Western blot and immunohistochemistry. Differential methylation pattern of genes in CHM and normal early chorionic villi were examined by MeDIP-chip (methylated DNA immunoprecipitation-chip)， analysised and functional charactered by bioinformatics. The results showed mRNA expression (P=0.0083) of MBD2 was significantly higher in CHM than that in normal chorionic villi (P<0.01). The protein level was coincident with mRNA level when examined by Western blot (P=0.0005， P<0.01) and immunohistochemistry (P=0.0091， P<0.01). MeDIP-chip results showed 89 genes in CHM were demethylated， of which 85 genes can be mapped to genome. Our results suggested that over-expression of MBD2 and related gene demethylation may play vital roles in the genesis of CHM.
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