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Chromosomal Instability and Kinetochore Variation in HEP-2 Cells
Chun-Lan Long, Bin Tan, Xue-Qing Liu, Yu-Bin Ding, Xue-Mei Chen,Qiu-Bo Yu, Ru-Fei Gao, Ying-Xiong Wang, Jun-Lin He*
Department of Genetics, Chongqing Medical University, Chongqing 400016, China
Abstract: Chromosomal instability is a marked character in many tumor cells， however the mechanism of this instability has not been clarified up to now. In this study， kinetochore variation of HEP-2 cells were analysized by an improved method for staining kinetochores of human chromosomes in order to probe the mechanism of chromosomal instability of tumor cells. The metaphases of 308 (including 16 962 scored chromosomes) from HEP- 2 cells and 300 (including 13 800 scored chromosomes) from normal control were analyzed under microscope. The results showed that: 1) compared to normal cells， frequency of kinetochore loss， kinetochore duplication laggard and kinetochore dissymmetry in HEP-2 cells were significantly higher than that of control respectively (P<0.01). 2) a cytogenentic phenomenon of multi-kinetochores was observed in HEP-2 cells， and we suggested that it might be a new mechanism for inducing chromosomal structure abnormality in tumor cell.
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