Cloning and Identification of Two Isoforms of ATP8A2， Member of Murine P Type ATPase

Abstract: Specific phospholipids are asymmetrically and dynamically distributed in the two leaflets of the eukaryotic membrane bilayers， by the activities of phospholipid translocation. However， the mechanisms and proteins involved in these processes are still poorly understood. Members of the type 4 subfamily of P-type ATPases have been implicated in the translocation of phospholipids from the outer to the inner leaflet of membrane bilayers， which we now call flippase activity. All of the 5 yeast type 4 subfamily of P-type ATPases have been demonstrated to have flippase activity as well as function in cell polarity establishment and vesicle traffic. In mammalian， the studies of flippase mostly focus on pathology， and little has been known about the molecular mechanism underlying these disorders. Here we cloned a murine P-type ATPases ATP8A2 which is an ortholog of DRS2p. Surprisely， we found it has two isoforms. The tissue expression pattern indicates ATP8A2， especially isoform α is mainly expressed in testis， providing cases for analysising its function during spermatogenesis.

CSTR: 32200.14.cjcb.2008.01.0015