RNAi Technique Based on Green Fluorescent Protein and Its Application in Course of Cell Biology Experiment

Abstract: RNAi and GFP gene transfer technique are commonly used in cell biology research field. With GFP as the target gene， we developed this RNAi experiment project that is suitable for undergraduate students. In this project， we integrated GFP fluorescence observation， gene transfer and RNA interference technique into one project. Also we designed its detailed protocol and did concrete practice for this experiment project. Results showed that RNAi technique could significantly inhibit the expression of GFP gene in cell， so we drew the conclusion that this experiment project had good feasibility and necessity to be carried out in cell biology experiment course for undergraduates. This cross-cutting， integrated experiment projects should have a good effect for students' integrated research ability， and also provide a novel idea for cell biology experiment course reform.

CSTR: 32200.14.cjcb.2010.06.0013