Study on the Mechanism about ATRA to Promote the Apoptosis of DDP on Lung Adenocarcinoma A549

Abstract: To discuss the effect of all-trans retinoic acid (ATRA) on the cisplatin (DDP) induced lung adenocarcinoma cell line (A549) apoptosis and its mechanism. Using methyl thiazolyl tetrazolium salt assay (MTT)， real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and flow cytometry to detect the A549 apoptosis rate， the transcription level of retinoic acid receptor β (RARβ) mRNA and the transcription level of inhibitor of apoptosis protein Survivin mRNA. The results showed that DDP had inhibition effect on A549 in a dosedependent way. When ATRA was less than 0.4 μmol/L， the inhibition effect was not obvious， but when it up to 0.4 μmol/L or higher concentrations， the inhibition effect was significantly in a dose-dependent way. The qRT-PCR results showed that the expression of RARβ mRNA and Survivin mRNA all decreased in the DDP groups. In ATRA groups the expression of RARβ mRNA increased， while the expression of Survivin mRNA decreased. In Combined treatment groups， the expression of RARβ mRNA was lower than that in the Control groups but higher than that in the DDP groups. And when it comes to Survivin mRNA， it decreased both in Control groups and DDP groups. The flow cytometry results showed that the apoptosis rate in combined treatment groups were higher than that in single drug groups. In conclusion， the ATRA could promote the expression of RARβ while inhibited the expression of Survivin. Through such means， ATRA could increase the chemosensitivity of A549 and strengthen the role of DDP chemotherapy.

CSTR: 32200.14.cjcb.2010.06.0012