Home > Browse Issues > Vol.32 No.6
Glutaraldehyde Induced Infrared Fluorescence for Detection and Quantitation of Protein in Polyacrylamide Gels
Gang He, Jing-Bao Li, Liang Wang, Peng Shang, Ai-Rong Qian*
Key Laboratory for Space Biosciences and Biotechnology, Institute of Special Environmental Biophysics, Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China
Abstract: Protein detection and quantitation is very important in biochemical analysis. Reaction of glutaraldehyde with proteins results in nonspecific infrared fluorescence at some excitation wavelengths， and the intensity of fluorescence was directly proportional to protein content over the range of 7.8 ng to 2 000 ng for our test protein， BSA. We took advantage of this property to develop a new method for detection and quantitation of proteins in polyacrylamide gels. The staining can be performed at room temperature， but is faster when gels exposure to microwave. Staining requires only 3~5 min， don’t need destaining process. The method can be used for routine analytical purposes due to its sensitivity， speed and simplicity.
CN
EN