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Culture and Identification of ES-like Cells from Chicken Embryonic Ovarian
Li-Ying Geng1, Chuan-Sheng Zhang1*, Chun-Guang Yin2, Li-Xin Du3*, Zheng-Zhu Liu1, Zhi-Xin Fu1, Jing-Rui Yan1
1College of Life Science, Hebei Normal University, Changli 066600, China; 2Life Sciences and Engineering Department of Jining University, Jining 273155,China; 3Institute of Animal Science, Chinese Academy of Agricultural Science, Bei
Abstract: This paper attempts to explore the feasibility of isolation and culture the ES cells from chicken embryonic ovarian gonocytes cells. The ES-Like cells were detected when the 18d chicken embryonic ovarian cells of were cultured in high glucose DMEM medium supplemented with LIF， bFGF， SCF， IGF and GDNF for primary culture and subcultured with STO feeder. The colonies had the morphology of nest-like and were positive for periodic acid-Schiff and alkaline phosphatase staining. They expressed the pluripotent markers such as SSEA-1， SSEA-4， TRA-1-60 and TRA-A-81. Furthermore， the ES-Like cells could form embryoid bodies and differentiate to all kinds of cell types in vitro. It seems that these cells also maintained characters of pluripotent stem cells.
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