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Temporal and Spacial Expression Analysis of MSTN-1 and -2 in Pseudosciaena crocea
Liang-Yi Xue*, Sheng Sun
College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
Abstract: Myostatin (MSTN) is an important negative regulator in animal muscle growth and development. Large yellow croaker， Pseudosciaena crocea， has two types of MSTN genes， MSTN-1 and -2. Embryonic and tissuespecific expressions of MSTN-1 and -2 genes were analyzed by using RT-PCR. In 6 and 18-month old fish， both MSTN-1 and -2 genes expressed in a wide variety of tissues. However， only MSTN-1 expressed in muscle tissue. In embryonic development， MSTN-1 expressed before blastula stage (6 hpf)， and was undetected throughout gastrulation (9hpf)， and rised again in hatching (27 hpf). MSTN-2 was undetected through embryogenesis. These results indicated that the expression of MSTN-1 and -2 genes was differentially regulated in both developing embryos and adult tissues of fish.
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