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Effects of Tongluo Recipe on Proliferation and Oxidative Stress of Rat Glomerular Mesangial Cells under High Glucose
Lan-Yu Zhang, Jun-Jie Zou, Xiao-Yun Feng, Zhi-Min Liu*
Department of Endocrinology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Abstract: To investigate the effects of Tongluo Recipe (TLR) on proliferation and oxidative stress of rat glomerular mesangial cells under high glucose， Rat mesangial cell lines (rat mesangial cells ，RMCs) under high glucose condition were treated with TLR. The proliferation of mesangial cells was detected by MTT assay.The reactive oxygen was detected by DCFH-DA probes. The expression of mRNA of CuZnSOD， CAT， Gpx， MnSOD and FN were detected by RT-PCR and the protein of MnSOD and FN were detected by Western blot. Tongluo Recipe significantly decreased the proliferation and ROS of rat mesangial cells under high glucose (P<0.05). Tongluo Recipe could increase the protein level of MnSOD under normal glucose conditions and decrease the protein level of Fibronectin (FN) in a dose-dependent manner. So the Tongluo Recipe could inhibit the proliferation， inhibit the generation of ROS and improve the antioxidative enzymes capacities of rat glomerular mesangial cells under high glucose.
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