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Protection Effect of Plantain Seed Polysaccharid on Human Umbilical Vein Endothelial Cells Injury Induced by Oxidized Low Density Lipoprotein
Wen-Wen Che1,2, Li-Hong Duan3, Ning Zhang1, Na Feng1, Su-Min Wang1*
1Pharmacology Department, Basic Medical College, Hebei Medical University, Shijiazhuang 050017, China; 2Jinan 106 Hospital, Jinan 250022, China; 3Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang 050011,China
Abstract: To investigate the protective effect of PSP on the oxidized low density lipoprotein-induced lipid peroxidation injury HUVECs model and study possible mechanism， ox-LDL-induced injury HUVECs were used. We used different experimental methods to analyze the following parameters: proliferation rate， NO content， NOS activity， MDA content， SOD vitality of HUVECs by MTT assay and chemical methods in the cellular level; ICAM-1 expression and apoptosis-related gene mRNA was assessed by ELISA and RT-PCR in the molecular level. Finally， we observed that different concentrations of PSP decreased the ox-LDL-induced MDA content， and increased the ox-LDL-induced SOD， NO and NOS activity and the expression of ICAM-1 by ELISA， c-myc mRNA and p53 mRNA by Polymerase Chain Reaction (PCR)， which indicates PSP had the protective effect of ox-LDL-induced endothelial cell injury， which is related to the down-regulated expression of ICAM-1， c-myc mRNA and p53.
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