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Necrosis， Not Apoptosis of RAW264.7 Mrophages Following Infection with GAS
Cui-Qing Ma, Guang-Lei Hu, Jian-Ling Yang, Jie Hu, Ling Zhang, Hui-Dong Feng, Li-Juan Yang, Lin Wei*
Department of Immunology, Basic Medical College, Hebei Medical University, Shijiazhuang 050017, China
Abstract: To study the proliferation of macrophage RAW264.7 infected with group A streptococcus (GAS)， exploring the pathogenic mechanism of GAS， RAW264.7 cells were seeded into 96 plates， and then infected with GAS at an MOI (multiply of infection) ranging from 5:1 to 100:1 (bacteria number per macrophage) for 20 min， 40 min， 60 min， 90 min and 120 min， subsequently the bacteria were abandoned and the infected RAW264.7 cells were incubated for 3d， and then added MTT to measure OD490. Following high-dose GAS stimulation， apoptosis was detected by TUNEL and ANNEXIN V-FITC. The ultrastructure of RAW264.7 cells， infected with GAS， was observed by transmission electron microscopy. We found that the activation and proliferation of RAW264.7 cells were enhanced following infections with low-dose GAS， while the proliferation of cells was declined when cells were infected with high-dose GAS， moreover， the cells were found to be necrosis but not apoptosis. Our results suggest that macrophages RAW264.7 are induced to be necrosis but not apoptosis with high-dose GAS simulation.
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