Research and Application of Animal Transgenic Technology

Abstract: The animal transgenic technology which originated from the 70s~80s of the 20th century was a kind of advanced biological technology. It foreshowed a broad prospect for application as its birth. After years of development， the transgenic technology had formed many mature methods， such as microinjection method， retroviral vector method， sperm vector method， somatic cell nuclear transplantation method and so on. There were also many new methods emerged in recent years. They were gonadal injection method， RNAi method， combination of gene targeting with cloning and so on. In this paper， we mainly summarized the basic principles of various kinds of transgenic technologies and their latest research trends at home and abroad on the basis of previous studies， besides， we also discussed the advantages and disadvantages of different kinds of transgenic methods in detail. At last， we described the application of transgenic technologies in livestock. Apart from this， we also analyzed the problems in transgenic technologies.

CSTR: 32200.14.cjcb.2010.05.0022