Home > Browse Issues > Vol.32 No.5
Atomic Force Microscopy in Nano-biological Study
Qing-Qing Jiang1, Li-Juan Zhang2, Yu Zhang1, Yan Guo1, Wei Ren1, Xiao-Hui Zhang1, Xin-Yan Wang1*
1Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, CAS, Shanghai 200031, China;2Chongqing academy of animal science, Chongqing 402460, China)
Abstract: Since its invention in 1986， the atomic force microscope (AFM) has emerged as a flexible and powerful tool for exploring a variety of biological processes， including cell adhesion， protein folding， and protein– protein interactions. This review focuses on the application of the AFM to studies of protein–protein interactions. It describes the commonly used methodologies and reviews the theoretical framework used to analyze single-molecule protein–protein unbinding measurements. This review summarizes recent progress in the field and shows that the AFM provides an excellent tool for probing interactions on the cell surface， for understanding the energy landscapes that govern the dynamics of protein interactions， and the common used method of surface modification.
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