Home > Browse Issues > Vol.32 No.5
Study on the Mobilization of Peripheral Blood Mesenchymal Stem Cells from Rat by G-CSF
Xiao-Chun Sun*, Hua Cai, Le-Le Wu, Wen-Rong Xu, Hua-Xi Xu, Qiao-Lin Chen
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013,China
Abstract: To isolate and culture the mesenchymal stem cells (MSCs) of rat from peripheral blood and marrow， granulocyte colony-stimulating factor(G-CSF) was used. The percentage of CD44+ cells increased significantly higher after mobilization by G-CSF. MSCs were isolated and cultured unsuccessfully from peripheral blood control group and mobilized I group (mobilizated by 20 μg/kg G-CSF). The typical colonies of MSCs were isolated after being mobilization by 50 μg/kg and 80 μg/kg G-CSF. Surface markers of obtained MSCs were accordance with MSCs from bone marrow by FACS assay. We confirmed that the peripheral blood MSCs from rat could be mobilizated dependent on the dosage of G-CSF.
CN
EN