Compariation of Tissue Explant and Enzyme Digestion Method for Rat Vaginal Epithelial Cells in vitro

Abstract: Vaginal epithelial cells from Sprague-Dawley rats were primary cultured by tissue explant and enzyme digestion method respectively to explore the better culture technique in vitro and provide seed cells for engineering of vaginal tissue. Time of primary culture， the cell morphology and proliferation characteristics were oberserved under light microscopic. The cells were identified with P-CK anti by immunohistochemistry staining. The results indicated that the vaginal epithelial cells which were obtained by the two methods were polygon-like or irregular globular， and their morphology and growth characteristics were essentially consistent. The expression of pan-cytokeratin staining of the cultured cells were positive. However， compared with explant method， the culture cells using enzyme digestion method displayed more faster adhesion， shorter growth time， and higher cells productivity and purity. The larger number of epithelial cells were rapidly obtained to construct engineering of vaginal tissue by enzyme digestion method.

CSTR: 32200.14.cjcb.2010.05.0014