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Rho-GDIα Inhibited the Migration of Vascular Smooth Muscle Cells Induced by Nicotine in Rats
Ming-Juan Qu*, Fei-Fei Shu, Mei-Dong Jing, Li Zhang
College of Life Sciences, Ludong University, Yantai 264010, China
Abstract: To investigate the mechanism of the smoking-induced progress and mechanism of the atherosclerosis， the effect of nicotine on the migration of vascular smooth muscle cells (VSMCs) of rats was studied. Vascular smooth muscle cells (VSMCs) isolated from rats were subjected to 100 μmol/L nicotine for 24 hours， and cells treated without nicotine were used as control. The migration ability of VSMCs was checked by Transwell assay and Wound healing assay， respectively. The mRNA expression of Rho-GDIα was detected using RNA interference (RNAi) and Real-time RT-PCR. The migration ability of VSMCs was significantly promoted by nicotine stimulation compared with the control group. The mRNA expression of Rho-GDIα was depressed by nicotine treated as well as RNAi. It is concluded that nicotine can promote the migration of VSMC in vitro partly through inhibiting the expression of Rho-GDIα， which explicits the role of nicotine in the atherosclerosis.
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