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Effect of NPM1 Mutations on the Invasion Potential of Leukemic Cells in vitro
Hui-Yuan Shao, Zong-Yu Miao, Feng-Xian Qin, Xian-Chun Chen, Shi Tan, Yu-Jie Gao, Ling Zhang*
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
Abstract: Nucleophosmin (NPM1) mutations have been recently identified as the most frequent genetic alterations in acute myeloid leukemia and are relationship with leukemiagenesis. To explore the role of NPM1 mutations in the invasion of leukemia， the pEGFPC1-NPM1-mA plasmid vector with NPM1 mutation A (NPM1-mA) was transfected into THP-1 cells， and the leukemic cells with stably expressed NPM1-mA protein (THP-1-mA) were established. Transwell migration assay， Matrigel invasion assay and cell adhesion assay were performed. These results showed that both invasion and migratory capacities of THP-1-mA cells were much higher compared with parent THP-1 cells. In addition， THP-1-mA cells displayed higher adhesion to fibronectin compared with THP-1 cells. Therefore， our findings indicate that NPM1 mutations enhance the invasion potential of leukemic cells in vitro， which makes a further understanding of NPM1 mutations in the malignant transformation of leukemic cells.
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