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Expression Decreasing of Protooncogene C-MET in Decidua of Spontaneous Abortion
Xue-Mei Chen, Jin-Yuan Huang, Xue-Qing Liu, Yu-Bin Ding, Ying-Xiong Wang, Jun-Lin He*
Laboratory of Reproductive Biology, College of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the role of proto-oncogene C-MET in decidua during embryo implantation and spontaneous abortion， we characterized the expression of C-MET mRNA and protein in decidua collected from 30 cases of normal pregnancy and 30 cases of spontaneous abortion. Real-time PCR and in situ hybridization results showed that the levels of C-MET mRNA in decidua of normal pregnancy were significantly higher than those of spontaneous abortion. The C-MET protein detected by immunofluorescence histochemistry showed the same tendency with C-MET mRNA and the protein mainly located in nucleus and endochylema of stromal cell. Further more， polyclonal antibody of C-MET was injected into the uterus horns of pregnant mice (d3) to investigate its effect on embryo implantation. The results showed that the number of implanted embryo markedly decreased in the side of uterus horns receiving polyclonal antibody of C-MET (P<0.05). These findings suggest that C-MET may play an important role in embryo implantation and low expression of C-MET may induce spontaneous abortion.
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