Home > Browse Issues > Vol.32 No.5
Expression of Oct-4 Transcriptional Factor Rregulates the Anti-Apoptosis Effect of Survivin in Hepatobiliary Cancer Cells
Duan-Ming Wang1, Xiao-Qing Jiang2, Wei-Feng Tan2, Chen Liu2, Jing-Han Wang2, Lin-Fang Li2,Qi-Jun Qian2, Chang-Qing Su2*
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; 2Eastern Hepatobiliary Surgical Hospital,Second Military Medical University, Shanghai 200438, China
Abstract: The transcriptional factor Oct-4 and Survivin are the key regulatory factors in cancer cell proliferation and mitosis. To investigate the relationship between Oct-4 and Survivin and the effect on genetic characteristics of cancer cells， the expressions of Oct-4 and Survivin were examined immunohistochemically in the clinical specimens of primary hepatocellular carcinoma， gallbladder carcinoma and cholangiocarcinoma. The positive rates of Oct-4 and Survivin were 60.7% and 75.0%， respectively， and there was a parallel relationship between Oct-4 and Survivin in the hepatobiliary cancers. The cancer cell lines of primary hepatocellular carcinoma， gallbladder carcinoma and cholangiocarcinoma， EHBH-H1， EH-GB1 and EH-CA1， were established. The expression of Survivin in EH-CA1 was silenced by adenovirus carrying Survivin-shRNA， and the re-expression of Oct-4 in EHGB1 was done by adenovirus carrying Oct-4 cDNA. The cell cycle and apoptosis were observed by flow cytometry. The results demonstrated that Oct-4 could regulate and enhance the expression of Survivin， then promote the cell cycle progression and inhibit cell apoptosis in cancer cells. This finding provides us a novel target for cancer gene therapy.
CN
EN