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Applications of Total Internal Reflection Fluorescence Microscopy for Single-molecule Detection in Living Cells
Xuan Zhou, Lei Wang*
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
Abstract: Total internal reflection fluorescence microscopy (TIRFM) is a sensitive and fast singlemolecule detection and imaging technique. It has been successfully applied to the fields of life science， chemistry and physics， particularly the area of single-molecule detection in living cells. In this paper， we reviewed the principle of TIRFM and the applications of TIRFM for single-molecule detection in living cells. We also prospected the development of TIRFM.
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