The Effect of Different Cells Density on the Bovine Mammary Epithelial Cells Cryopreservation

Abstract: In this study， the breast tissue of a healthy Holstein dairy cow was used. Through five-generation purification， the bovine mammary epithelial cells were divided into four groups， according to the cell density (1×104 cells/ml， group 1; 1×105 cells/ml， group 2; 1×106 cells/ml， group 3; 1×107 cells/ml， group 4 respectively) and cryopreservated， then thawed after 1 month and maintained separately. The results showed that group 4 had the lowest death rate， significantly lower to other three treatments (P<0.01)， and the highest adhesion rate， significantly higher to other three treatments (P<0.05). Group 3 had the lowest apoptosis rate， significantly lower to other three treatments (P<0.05). The cells of group 3 and group 4 basically covered with the bottom of the culture flask and the cells were in good condition at three days after adherence.

CSTR: 32200.14.cjcb.2010.04.0020