[¹³C]-Octanoic Acid Breath Test in Detection of Gastric Emptying of the Laboratory Mouse

Wei-Gang Wang¹, Jia-Bin Zhou², Ying-Han Wan², Hui-Min Yan^2*

¹School of Life Science and Technology, Tongji University, Shanghai 200092, China; ²Shanghai Research Center for Biomodel Organism, Shanghai 201203, China

Abstract: Gastric emptying studies in the laboratory mouse are hampered for the lack of a non-invasive and repeatable technique. The aim of the present study was to adapt the non-invasive [13C]-octanoic acid breath test to assess gastric emptying of both liquid and solid meal in the mouse. Gastric emptying rates were investigated for a liquid meal (Intralipid) and a solid meal (egg yolk) incorporating [13C]-octanoic acid. Mathematical analysis of the 13CO2 excretion rate allowed the calculation of gastric emptying parameters (GEC， t1/2， and tlag). The breath test can continuously and non-invasively assess gastric emptying in the mouse. This method can be used to assess gastric emptying in drug development and disease studies in the mouse.

CSTR: 32200.14.cjcb.2010.04.0019

[13C]-Octanoic Acid Breath Test in Detection of Gastric Emptying of the Laboratory Mouse

[¹³C]-Octanoic Acid Breath Test in Detection of Gastric Emptying of the Laboratory Mouse