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NDGA Up-regulates the Expression of p27 and p53 in Cervical Cancer SiHa Cells
Peng Gao, Lei Guan, Fei Zhai, Jie Zheng*
Department of Pathology and Pathophysiology, School of Medical Science, Southeast University, Nanjing 210009, China
Abstract: Our previous study showed that nordihydroguaiaretic acid (NDGA) treatment leads to cell cycle arrest at G1 for elevated p21 which is mediated by elevated level of histone H3 acetylation in cervical cancer SiHa cells. Here， we further detected the effect of NDGA on two other cell cycle regulators: p27 and p53. Results showed that NDGA could up-regulate p27 both at transcription and protein levels， however， NDGA had no effect on acetylation level of histone H3 associated with p27 promoter. NDGA also up-regulated p53 protein level by inhibiting transcription of HPV-16 E6. These results suggest that NDGA can arrest cervical cancer cells at G1 relating to upregulating p27 and p53 levels， and the latter is associated with the inhibitory effect of NDGA on HPV-16 E6.
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