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Screening the Interaction Protein of DAXX by Yeast Two-hybrid System
Fang Liu1, Zhi-Yin Du2, Qiu-Bo Yu3, Ying-Xiong Wang4*
1Department of Clinical Laboratory, the First People`s Hospital in Jiangbei District, Chongqing 400020,China; 2Faculty of Information Management, Chongqing University of Medical Science, Chongqing 400016, China; 3College o
Abstract: To study interaction proteins of DAXX by yeast two-hybrid system， we use the full length of DAXX as bait to screen the human liver cDNA library and to find the proteins interacting with DAXX. The false clones were eliminated by bioinformatics methods and one to one yeast two-hybrid system. Three true positive clones from yeast clones were obtained after verification. The encoding proteins we obtained may play important roles in finding the new function of DAXX.
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