Bisphosphonates Preliminary Study of Tumor Suppressor Mechanism

Abstract: Recent studies showed that many cancer cell lines could be inhibited by BPs. We used the ibandronate (IB) and CP on the human gastric adenocarcinoma cell line (SGC-7901) to further explore the mechanism. The methods of MTT， cytometric analysis， immunocytochemical staining and Western blot were employed to evaluate the influence of BPs on the proliferation， apoptosis and cycle of tumor cells and the expression of IL-6， p- STAT-3 protein in human gastric adenocarcinoma cell line (SGC-7901). The result showed that SGC-7901 cell proliferation could be significantly inhibited by BPs， and the effect of CP was more significant than that of IB. FCM suggested that BPs stimulated apoptosis of SGC-7901 cells and inhibited cells on S phase， while S fraction cells increased in treated groups in a dose and time-dependent manner， the effect of CP was more significant than that of IB. The result of immunocytochemical staining and Western blot showed that the relative content of IL-6 protein and p-STAT-3 protein in BPs treated group was lower than that in the control group; the effect of CP was more significant than IB. The anti-tumor mechanism of BPs was protecting the stabilization of signal transducer and activator of transcription， then affecting the proliferation and apoptosis of cancer cells by down-regulate protein expression of IL-6 and p-STAT-3.

CSTR: 32200.14.cjcb.2010.04.0016