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Analysis of microRNA Expression Patterns in Pulmonary Arterial Hypertension Associated with Congenital Heart Disease
Wei-Dan Chen, Zhong-Dong Hua, Ke-Ming Yang, Qiang Meng, Hao Zhang, Ying Zhang, Xiang-bin Pan, De Wang, Yan Li, Shou-Jun Li*
Pediatric Cardiac Surgery Center, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
Abstract: It has been found that several miRNAs play a great role in cardiovascular system recently. To investigate the expression pattern of microRNA (miRNA) in pulmonary arterial hypertension (PAH) associated with congenital heart disease (CHD) by miRNA array， lung tissues were collected from patients with severe PAH due to ventricular septal defect (VSD) (PAH group， n=6) and patients with VSD but without PAH (control group， n=6). Total RNA was extracted from tissues collected and the different expression of miRNA was detected by miRNA array and also verified using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Among 1 468 target miRNAs， 78 miRNAs showed different expression levels， 62 miRNAs were up-regulated and 16 miRNAs were down-regulated. In conclusion， miRNA may become a new target of PAH reversing and preventing drug since it is involved in the development of PAH associated with CHD.
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