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Pseudopod Extension and Retraction of Human Neutrophils in Response to Uniform Concentration of fMLP
Qing-Yi Wu1, Chun-Qing Cai1, Cui-Hua Xie2, Chun-Hua Yuan1, Xiao-Jing Meng1*
1Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China; 2Department of Endocrinology and Metabolism,Nanfang Hospital, Guangzhou 510515, China
Abstract: To analyze the polarization rate and the changes of pseudopod length in neutrophil， Percoll density gradient centrifugation upon the stimulation of 10-7~10-8 mol/L N-formyl-methionyl-leucyl-phenylalanine (fMLP) was used. The polarization rate of neutrophil was significantly increased after treated by 10 nmol/L， 50 nmol/L and 100 nmol/L fMLP separately in 4min， and reached 90% from 3 min to 4 min in all three groups. There was fastest increase of the polarization rate in the group of 100 nmol/L fMLP treatment. The pseudopod length of neutrophil was affected markedly by stimulation of the different concentrations of fMLP， which showed an oscillatory phenomenon of the pseudopod extention and retraction and an oscillatory cycle was formed. The oscillatory frequency and the oscillatory cycles were significantly affected by stimulation of the different concentration of fMLP. The neutrophils were classified by their activity of polarization in this study according to the change rate of peseudopod. The change of pseudopod was represented by F-actin polymerization which was modulated by polarization signaling molecule. The analysis of the pseudopod change could contribute to the knowledge of the signaling mechanism in neutrophil polarization.
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