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Effect of Troglitazone on Gene Expression and Secretion of Adipocytokines during Human Omental Preadipocytes Differentiation
Jin-Hua Yin1, Jing Yang1, Ming Li2*
1First Affiliated Hospital, Shanxi University of Medical, Taiyuan 030001, China; 2Peking Union Medical College Hospital, ChineseAcademy of Medical Sciences, Beijing 100730, China
Abstract: Omental preadipocytes were primarily cultured and interfered by collagenase digestion. Troglitazone intervention cells， oil red O staining differentiation， ELISA determination of the level of secretion of adipocytokines， RT-PCR observation for the differentiation transcription factors and fat cell factor mRNA expression， the purpose is to study mechanism of thiazolidinediones from the perspective of the visceral fat storage lipid and endocrine. The results showed that troglitazone increased the human preadipocyte differentiation， improved visceral adipose tissue storage lipid capacity; it can significantly increase adiponectin secretion; regulate leptin secretion; inhibite resisting factor secretion. This suggested that thiazolidinediones improved insulin resistance and inflammation in the body by
regulating adipocytokines spectrum. The main mechanism is to promote PPARγ2， C/EBPα and lipoprotein lipase (LPL) of mRNA. The increase in fat cells and changes in leptin secretion may partly explain the negative effects of
thiazolidinediones in increasing body weight.
regulating adipocytokines spectrum. The main mechanism is to promote PPARγ2， C/EBPα and lipoprotein lipase (LPL) of mRNA. The increase in fat cells and changes in leptin secretion may partly explain the negative effects of
thiazolidinediones in increasing body weight.
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