{"code":0,"msg":"","count":6092,"data":[{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"武汉大学生命科学学院, 武汉430072","aop":"","author":"张 煜 钟 波 杨 艳 舒红兵﹡","cabstract":"Toll样受体(Toll-like receptors，TLRs)和RIG-I样受体(RIG-I like receptors，
RLRs)为宿主细胞识别病毒的两种重要模式受体(pattern recognition receptors，RRs)。前者定位于细胞膜组分，后者定位于胞浆，从而识别通过不同途径产生的病毒核酸， 并通过招募特异接头蛋白，激活一系列信号级联反应，引发I型干扰素和促炎症细胞因子的产生。同时，宿主细胞通过各种方式调节TLRs和RLRs介导的信号通路，从而保证信号转导的稳态，并防止过度免疫反应对宿主造成伤害。此外，某些病毒也能通过其自身的结构蛋白或非结构蛋白，阻断TLRs和RLRs介导的信号通路，从而逃逸宿主对其识别与清除。本文将重点讨论TLRs和RLRs介导I型干扰素产生的信号转导及其调节机制， 并简要介绍病毒对信号转导的调节。","caddress":"Tel: 027-68753795; E-mail: shuh@whu.edu.cn","cdoi":"32200.14.cjcb.2009.04.0001","content1":"","csource":"","ctype":"特约综述","ctypeid":1,"doi":"10.11844/cjcb.2009.04.0001","eabstract":"Toll-like receptors (TLRs) and RIG-I like receptors (RLRs) are two classes of pattern recognition receptors (PRRs) in host cells. TLRs are located at the membrane system of cells， while the RLRs are cytoplasmic viral sensors. Upon reognition of the PAMPs generated by viruses during their invasion and replication， TLRs and RLRs recruit various of adaptors and activate a series of signaling cascades， leading to induction of type I interferons (IFNs) and proinflammatory cytokines. Meanwhile， host cells also adopt a number of strategies to regulate TLRs-/RLRs-mediated signaling to prevent harmful excessive immune responses. In addition， many viruses also regulate TLRs- and/or RLRs-mediated signaling through their structural or non-structural proteins， thereby escaping the recognition and clearance by the host. In this review， we focus on the mechanisms and regulation of TLRs- and RLRs-mediated signaling， as well as a brief introduction of virus-mediated regulation of TLRs- and RLRs-mediated signaling.
","eaffiliation":"College of Life Sciences, Wuhan University, Wuhan 430072, China","eauthor":"Yu Zhang, Bo Zhong, Yan Yang, Hong-Bing Shu*<\/sup>","ecauthor":"Tel: 86-27-68753795; E-mail: shuh@whu.edu.cn","ekeyword":"TLRs; RLRs; RNA virus; signaling transduction; negative regulation ","endpage":468,"esource":"","etimes":1477,"etitle":"Mechanisms and Regulations of TLRs- and RLRs-mediated Cellular Antiviral Signaling","etype":"INVITED REVIEWS","etypeid":5,"fundproject":"","keyword":"Toll样受体; RIG-I样受体; RNA病毒; 信号转导; 负调控","netpublicdate":"2009-10-28 15:33:53","pdfile1":"Upload/volpdf/20141029-200904001.pdf","seqno":"2","startpage":453,"status":"1","times":3860,"title":"TLRs与RLRs介导的细胞抗病毒反应信号转导及其调节机制","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"重庆大学生物工程学院, 重庆400044","aop":"","author":"杨本艳姿 王红兵* 杨 力 吴泽志","cabstract":"肌球蛋白轻链激酶(myosin light chain kinase， MLCK)和ROCK/ROK/Rho激酶(Rho kinase， ROCK)是肌细胞和非肌细胞中调节肌球蛋白轻链磷酸化的两种重要激酶，
肌球蛋白轻链磷酸化调节肌球蛋白的收缩参与了诸如细胞运动、粘附、组织修复和癌症转移和疾病发生等重要的生命活动。在这些重要的生命活动中， 同样是催化肌球蛋白磷酸化的这两种激酶， MLCK和ROCK定位在不同的细胞区域， 以不同的作用方式通过对细胞骨架的影响实现对细胞功能的精细调控。
","caddress":"Tel: 023-66885061, E-mail: whbdzx@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0002","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0002","eabstract":"Myosin light chain kinase and Rho kinase are two major enzymes controlling myosin light chain (MLC) phosphorylation in both muscle cells and non-muscle cells. MLC phosphorylation is the key reaction to regulate myosin contraction， which involved in many vital progresses， such as cell migration， adhesion， tissue repair， cancer metastasis and disease development etc. Although both of these two kinases could phosphorylate MLC and regulated cytoskeleton reorganization， there are distinct differences between their special location and the way to regulate cell function precisely by the cytoskeleton arrangement.","eaffiliation":"College of Bioengineerin , Chongqing University, Chongqing 400044, China","eauthor":"Yan-Zi Yangben, Hong-Bing Wang*<\/sup>, Li Yang, Ze-Zhi Wu ","ecauthor":"Tel: 86-23-66885061, E-mail: whbdzx@yahoo.com.cn","ekeyword":"myosin light chain kinase; Rho kinase; myosin light chain phosphorylation; cytoskeleton ","endpage":475,"esource":"This work was supported by the National Natural Science Foundation of China (No.30870608) and the 111 Project (No.B0623) ","etimes":1482,"etitle":"MCLK and ROCK Influence on Cytoskeleton and Cell Behaviors","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"ROCK; MLCK; 肌球蛋白轻链磷酸化; 细胞骨架","netpublicdate":"2009-10-28 16:22:36","pdfile1":"Upload/volpdf/20141029-200904002.pdf","seqno":"8","startpage":469,"status":"1","times":3270,"title":"MLCK和ROCK对细胞骨架和细胞行为的影响","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"首都师范大学生命科学学院, 北京100048","aop":"","author":"邱 鸿 于 荣*","cabstract":"微管和微丝作为构成细胞骨架的两大主要组分，通过多种相关蛋白在组成结构和信号调控等方面紧密作用， 在细胞中形成一个整体的网络系统。本文综述了微管和微丝在细胞迁移、细胞凋亡、胞质分裂、形态建成、胞质环流、细胞器运动和物质运输等生命活动中的相互作用。","caddress":"Tel: 010-68901692, E-mail: qiuhong666@126.com","cdoi":"32200.14.cjcb.2009.04.0003","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0003","eabstract":"As two major components of cytoskeleton， microtubules and microfilaments are interconnected in functions of structure and signal control by kinds of cross-bridges， and to be an integrated network. Here we showed that they cooperate functionally during a wide variety of processes， including cell migration， apoptosis， cytokinesis， morphogenesis， cytoplasmic streaming， organelle movement and cellular transport.","eaffiliation":"College of Life Sciences, Capital Normal University, Beijing 100048, China","eauthor":"Hong Qiu, Rong Yu*<\/sup> ","ecauthor":"Tel: 86-10-68901692, E-mail: qiuhong666@126.com","ekeyword":"microtubule; microfilament; interaction ","endpage":480,"esource":"This work was supported by the National Natural Science Foundation of China (No.30600318, No.30400228)","etimes":1473,"etitle":"The Interactions of Microtubules and Microfilaments","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"微管; 微丝; 相互作用","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200904003.pdf","seqno":"3","startpage":476,"status":"1","times":3576,"title":"微管和微丝的相互作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"中国科学院上海微系统与信息技术研究所纳米技术研究室, 上海 200050; 1<\/SUP>复旦大学生命科学学院, 上海200433","aop":"","author":"翟万银 周双雯1<\/SUP> 贾春平 徐元森*","cabstract":"藤黄酸是传统中药藤黄中的重要成分。近年来，由于它显著的抗癌作用和低细胞毒性，受到越来越多研究人员的关注。目前细胞生物学作用机制研究在(1)藤黄酸及其衍生物对肿瘤细胞增殖的抑制和促凋亡作用，(2)激活T淋巴细胞诱导肿瘤细胞凋亡，以及(3)肿瘤血管生成的抑制作用等方面取得一系列重要进展。虽然其作用的分子机制有可能尚未被完全揭示， 但这些机制为后续研究及临床应用打下了良好的基础。","caddress":"Tel: 021-62511070-801, Fax: 021-62511070-8714, E-mail: zhaiwy@mail.sic.ac.cn","cdoi":"32200.14.cjcb.2009.04.0004","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0004","eabstract":"Gambogic acid is the major active component isolated from the gamboge resin of Garcinia hanburyi tree. In recent years， its prominent anti-tumor activity and low side effect has aroused widespread interest among scholars. Its anti-tumor mechanisms have been elucidated in (1) inhibition on tumor cell proliferation and induction of apoptosis， (2) activation T lymphocytes which stimulate apoptosis of tumor cells， and (3) inhibition on tumor angiogenesis. Although the molecular mechanism of its anticancer activity remains insufficient understood， its achievements have paved its way to the research in future and clinical application.
","eaffiliation":"Lab of Nanotechnology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences,Shanghai 200050, China; 1<\/sup>School of Life Science, Fudan University, Shanghai 200062, China","eauthor":"Wan-Yin Zhai, Shuang-Wen Zhou1<\/sup>, Chun-Pin Jia, Yuan-Sen Xu*<\/sup> ","ecauthor":"Tel: 86-21-62511070-801, Fax: 86-21-62511070-8714, E-mail: zhaiwy@mail.sic.ac.cn","ekeyword":"gambogic acid; tumor; apoptosis; angiogenesis ","endpage":485,"esource":"This work was supported by the National High Technology Research and Development Program of China (863 Program) (No.2006AA03Z334), the National Fundamental Fund Project Subsidy Funds of Personnel Training (No.J0630643), and the Special Research Program in","etimes":1568,"etitle":"The Cytobiological Anti-cancer Mechanisms of Gambogic Acid","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"藤黄酸; 肿瘤; 凋亡; 血管生成","netpublicdate":"2009-10-28 15:56:30","pdfile1":"Upload/volpdf/20141029-200904004.pdf","seqno":"4","startpage":481,"status":"1","times":3296,"title":"藤黄酸抗肿瘤细胞生物学机制","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"广东药学院基础学院基础医学研究所, 广州510006; 1<\/SUP>中国科学院上海生命科学研究院生物化学与细胞生物学研究所, 上海200031","aop":"","author":"吴 婷 王伟章 耿建国1<\/SUP> 王丽京*","cabstract":"Slit/Robo信号作为神经导向因子在神经系统发育中具有导向轴突生长和排斥神经细胞迁移的重要作用， 该信号还有抑制白细胞的趋化功能和在发育期吸引血管发生的作用。目前Slit/Robo的研究主要集中在中枢神经系统发育上， 在肿瘤中的作用尚无定论。本文从Slit/Robo信号在肿瘤中的表达情况、在肿瘤新生血管和细胞迁移中的作用及其可能介导的信号通路等方面做一综述， 探讨Slit/Robo信号作为肿瘤治疗靶点的潜在意义， 对其深入研究具有重要的理论和临床意义。","caddress":"Tel: 020-39352126, E-mail: wanglijing62@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0005","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0005","eabstract":"Slit/Robo signal as neuronal guidance factor plays important roles in guiding axon growth and rejecting neuronal migration during the development of the nervous system. Furthermore， this signal also functions as a suppressor of leukocyte chemotaxis and an attractor of angiogenesis in development phase. Currently， most investigations focused on Slit/Robo signal in the central nervous system development. However， the role of this signal in carcinogenesis remained unknown. We review here the evidence for a role for Slit/Robo signal in cancer and focus on its role in tumor angiogenesis， cell migration and discuss the signaling pathways downstream of it， giving a new insight in the significance of Slit/Robo signal as a potential therapeutic target for cancer therapy.
","eaffiliation":"Institute of Basic Medical Sciences, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China; 1<\/sup>Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, S","eauthor":"Ting Wu, Wei-Zhang Wang, Jian-Guo Geng1<\/sup>, Li-Jing Wang*<\/sup>","ecauthor":"Tel: 86-20-39352126, E-mail: wanglijing62@yahoo.com.cn ","ekeyword":"neuronal guidance factor; Slit/Robo signal; tumor ","endpage":490,"esource":"This work was supported by the National Natural Science Foundation of China (No.30871307)","etimes":1524,"etitle":"The Role of Slit/Robo Signal in Tumor Development","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"神经导向因子; Slit/Robo信号; 肿瘤","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200904005 486.pdf","seqno":"5","startpage":486,"status":"1","times":3574,"title":"Slit/Robo信号在肿瘤发生发展中的作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"1<\/SUP>天津大学药物科学与技术学院, 天津 300072; 2<\/SUP>天津医科大学, 天津市基础医学研究中心, 天津300070","aop":"","author":"王继红1#<\/SUP> 李西川2#<\/SUP> 蒋伶活1,2<\/SUP>*","cabstract":"介绍了酵母ABC转运蛋白的分类和特征， 并对酵母ABC转运蛋白在多向耐药和脂类转运方面的功能进行了论述。酵母ABC转运蛋白研究的最新发现可能对ABC转运蛋白在真菌致病过程中的功能研究起到重要的理论指导意义。
#共同第一作者","caddress":"Tel: 022-27402527, Fax: 022-27401248, E-mail: linghuojiang@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0006","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0006","eabstract":"The classification and characteristics of ATP-binding cassette transporters (ABC transporters) in yeast are introduced， and recent progresses of functional studies on these ABC transporters in pleiotropic drug resistance and lipids trafficking are reviewed. Elucidation of functions of these ABC transporters might help us understand the mechanisms by which drug resistance occurs in pathogenic yeasts including Candida albicans.
","eaffiliation":"1<\/sup>School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China; 2<\/sup>Tianjin Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China","eauthor":"Ji-Hong Wang1#<\/sup>, Xi-Chuan Li2#<\/sup>, Ling-Huo Jiang1,2*<\/sup> ","ecauthor":"Tel: 86-22-27402527, Fax: 86-22-27401248, E-mail: linghuojiang@yahoo.com.cn ","ekeyword":"Saccharomyces cerevisiae; Candida albicans; ABC transporters; pleiotropic-drug-resistance ","endpage":496,"esource":"This work was supported by the National High Technology Research and Development Program of China (863 Program) (No.2007AA02Z187) and the National Natural Science Foundation of China (No.30870107) #These authors contribute equally to this work ","etimes":1511,"etitle":"Classification and Functions of ABC Transporters in Yeast","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"酿酒酵母菌; 白念珠菌; ABC转运蛋白; 多向耐药性","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200904006.pdf","seqno":"6","startpage":491,"status":"1","times":3665,"title":"酵母细胞中ABC转运蛋白的分类和功能","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"南京农业大学动物科技学院动物繁殖研究室, 南京210095; 1<\/SUP>日本国东京农工大学兽医系家畜生理学教室, 东京183-8509","aop":"","author":"曹 文 潘玲梅1<\/SUP> 田谷一善1<\/SUP> 石放雄*","cabstract":"卵母细胞成熟是一个包括内分泌、旁分泌以及自分泌的复杂过程， 所涉及的信号通路最终都要转化为对环核苷酸的调控。磷酸二酯酶(phosphodiesterase， PDE)是一类降解cAMP并使之失活的酶， 在卵巢中存在区域化分布。其中PDE3主要分布于卵母细胞， 而PDE4分布于颗粒细胞。体内外研究证实， 抑制哺乳动物卵母细胞PDE3活性能阻断减数分裂， 活性PDE3在两栖动物中的表达能诱导减数分裂重启， 程度与孕酮/胰岛素诱导的相近。PI-3K/Akt信号通路参与IGF1/胰岛素诱导的爪蟾卵母细胞成熟， 而在孕酮诱导的成熟中不起作用。PDE3作为PKB\\Akt激酶的下游因子， 通过调节胞内cAMP水平达到对卵母细胞成熟的调控。此外， 哺乳动物卵母细胞中也可能存在类似的信号通路。因此， PDE3在调控两栖类和哺乳动物减数分裂信号通路中扮演重要角色。掌握PDE3的调控方式便于人们更好的了解诱导卵母细胞成熟的信号通路。
","caddress":"Tel/Fax: 025-84395701, E-mail: fxshi@njau.edu.cn","cdoi":"32200.14.cjcb.2009.04.0007","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0007","eabstract":"Oocyte maturation is a complex set of endocrine， paracrine， and autocrine inputs that are translated into the regulation of cyclic nucleotide levels. Phosphodiesteras (PDEs)， the enzymes that degrade and inactivate cAMP， show compartmentalized expression in ovary. The PDE3 is mainly expressed in the oocytes while PDE4s are expressed in granulosa cells. Inhibition of the mammalian oocyte PDE3 completely blocked meiosis in vitro and in vivo， expression of an active PDE3A in Xenopus oocyte causes resumption of meiosis to the same extent as progesterone or insulin. PI-3K/Akt pathway mediates IGF-1/insulin but not progesterone-induced oocyte maturation. PDE3， as downstream factor of PKB/Akt， mediates oocyte maturation by controlling cAMP level. Furthermore， a similar regulatory module may exist in mammalian oocytes. Thus， PDE3 plays an essential role in the signaling pathway that controls resumption of meiosis in amphibians and mammals. Understanding the regulation of this enzyme may shed some light on the signals that trigger oocyte maturation.
","eaffiliation":"Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University,Nanjing 210095, China; 1<\/sup>Laboratory of Veterinary Physiology, Department of Veterinary Medicine,Tokyo University of Agriculture and Tech","eauthor":"Wen Cao, Ling-Mei Pan1<\/sup>, Kazuyoshi Taya1<\/sup>, Fang-Xiong Shi*<\/sup> ","ecauthor":"Tel/Fax: 86-25-84395701, E-mail: fxshi@njau.edu.cn","ekeyword":"cAMP; oocyte maturation; meiotic resumption; phosphodiesterase ","endpage":502,"esource":"This work was supported by the National Natural Science Foundation of China (No.30771553)","etimes":1506,"etitle":"Role of Cyclic Nucleotide Phosphodiesterases in Oocyte Maturation","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"环腺苷酸; 卵母细胞成熟; 减数分裂重启; 磷酸二酯酶","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200904007.pdf","seqno":"7","startpage":497,"status":"1","times":3313,"title":"磷酸二酯酶在卵母细胞成熟中的作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"石河子大学动物科技学院, 石河子832000; 1<\/SUP>新疆畜牧科学院农业部草食家畜繁育生物技术重点开放实验室, 乌鲁木齐830000","aop":"","author":"叶小芳 陈静波1<\/SUP> 黄俊成1<\/SUP>*","cabstract":"哺乳动物由于其卵子老化发生卵子质量下降， 从而导致老化卵母细胞受精后发育而来的胚胎非整倍体率增高， 妊娠流产率增加， 对后代产生了严重影响。研究表明一定剂量的抗氧化剂可以延缓卵母细胞的老化， 提高卵母细胞的质量， 抵抗卵子老化造成的负面影响。","caddress":"Tel: 0991-4835903, E-mail: h_jc@163.com","cdoi":"32200.14.cjcb.2009.04.0008","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0008","eabstract":"The poor quality of mammalian aged oocytes deteriorated which led to the ratios of heteroploid embryos and abortion ratio to increase and seriously affected the offspring. Research results showed that The appropriate dose of antioxidants could delay the aging process and improve the quality of the oocytes and relieve the negative influence caused by aging of oocytes","eaffiliation":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China; 1<\/sup> Key Laboratory of Reproduction & Breeding Biotechnology of Grass Feeding Livestock of Ministry of Agriculture, Xingjiang Academy of Animal Sciences, Urumqi 8","eauthor":"Xiao-Fang Ye, Jing-Bo Cheng1<\/sup>, Jun-Cheng Huang1*<\/sup> ","ecauthor":"Tel: 86-991-4835903, E-mail: h_jc@163.com ","ekeyword":"antioxidant; oocyte; aging; development ","endpage":508,"esource":"This work was supported by the Science and Technology Key Projects of Xinjiang Uygur Autonomous Region (No.200841122) and the China Postdoctoral Science Foundation ","etimes":1604,"etitle":"Antioxidants Delays Oocyte Aging","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"抗氧化剂; 卵母细胞; 老化; 发育","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200904008.pdf","seqno":"9","startpage":503,"status":"1","times":3306,"title":"抗氧化剂延缓卵母细胞老化","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"东北农业大学生命科学学院, 哈尔滨150030; 1<\/SUP>厦门市第一医院妇产科, 厦门361003","aop":"","author":"梁晓欢 邱娜旋1<\/SUP> 杨增明*","cabstract":"早期生长反应因子1(early growth response 1， Egr1)在多种刺激因素作用下都能被迅速上调， 参与细胞生长、增殖及凋亡等多种生理过程。作为转录因子， Egr1可结合到下游分子启动子区域的特定序列， 通过其下游分子发挥不同的功能。雌性生殖系统中， Egr1参与卵巢中卵泡发育及黄体发育过程， 调节乳腺细胞的增殖， 参与子宫中前列腺素合成， 调节转化相关蛋白53(transformation related protein 53， p53)等着床重要分子， 以及参与胎盘中血管发生等过程。Egr1在前列腺癌中也显著上调。本文综述了Egr1在哺乳动物生殖中的调节和功能。","caddress":"Tel: 0592-2186823, E-mail: zmyang@xmu.edu.cn","cdoi":"32200.14.cjcb.2009.04.0009","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0009","eabstract":"Early growth response 1 (Egr1) is upregulated in response to a wide variety of stimuli. Many biological roles have been attributed to Egr1， ranging from cell growth， proliferation as well as apoptosis. Once bound to the specific sequence in the promoter of target genes， Egr1 participates in regulating their expressions and executes various functions. Egr1 plays important roles in female reproduction through participating in follicular and luteal development， regulating the proliferation of mammary cells， involving in prostaglandin E biosynthesis in uterus， affecting the expression of p53 during implantation and contributing to angiogenesis in placenta. In male reproduction， Egr1 is highly expressed in prostate cancer. The regulation and function of Egr1 in mammalian reproduction were briefly reviewed.","eaffiliation":"College of Life Sciences, Northeast Agricultural University, Harbin 150030, China; 1Department of Obstetrics and Gynecology, First Hospital of Xiamen City, Xiamen 361003, China","eauthor":"Xiao-Huan Liang, Na-Xuan Qiu1<\/sup>, Zeng-Ming Yang*<\/sup>","ecauthor":"Tel: 86-592-2186823, E-mail: zmyang@xmu.edu.cn ","ekeyword":"early growth response 1; ovary; uterus; placenta; reproduction ","endpage":513,"esource":"","etimes":1491,"etitle":"Regulation and Function of Early Growth Response 1 during Mammalian Reproduction","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"早期生长反应因子1; 卵巢; 子宫; 胎盘; 生殖","netpublicdate":"2009-10-28 16:29:55","pdfile1":"Upload/volpdf/20141029-200904009.pdf","seqno":"10","startpage":509,"status":"1","times":3859,"title":"哺乳动物生殖过程中早期生长反应因子1的调节及功能","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"华中师范大学生命科学学院, 遗传调控与整合生物学湖北省重点实验室, 武汉430079","aop":"","author":"秦永芳 李登弟 李学宝*","cabstract":"同源异型-亮氨酸拉链(HD-Zip)蛋白是属于同源异型盒蛋白家族中为植物所特有的一类转录因子，它包含一个高度保守的同源异型结构域(HD)，HD羧基末端紧密连接着一个亮氨酸拉链(LZ)结构域。通过LZ结构域的相互作用，HD-Zip蛋白以二聚体的形式与靶DNA结合。HD-Zip蛋白在植物发育过程中的作用非常广泛，如维管发育、器官形成、分生组织的维持、逆境应答等。根据该类转录因子结合的特异性DNA序列，编码该类蛋白质的基因结构，包含的其他基序及其功能等四个方面的特征可以将HD-Zip蛋白分为I~IV四个亚类。本文对近年来有关四类HD-Zip转录因子生理功能的研究进展进行了综述。","caddress":"Tel/Fax: 027-67862443, E-mail: xbli@mail.ccnu.edu.cn","cdoi":"32200.14.cjcb.2009.04.0010","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.04.0010","eabstract":"Homeodomain-leucine zipper (HD-Zip) proteins， which display the singular combination of a highly conserved homeodomain and a leucine zipper acting as a dimerization motif， are transcription factors unique to plants. They are involved in a large number of processes (such as vascular development， organ formation， meristem maintenance， stress response， etc) in plants. Based on their four distinguishing characteristics (DNA-binding specificities， gene structures， additional conserved motifs and functions)， HD-Zip family can be divided into four subfamilies. In this review， recent progress in studying the function of HD-zip proteins was summarized.","eaffiliation":"Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China","eauthor":"Yong-Fang Qin, Deng-Di Li, Xue-Bao Li *<\/sup>","ecauthor":"Tel: 86-27-67862443, Fax: 86-27-67862443, E-mail: xbli@mail.ccnu.edu.cn ","ekeyword":" HD-Zip transcriptional factor; protein structure; classification; functions ","endpage":520,"esource":"This work was supported by the National Natural Science Foundation of China (No.30871317) ","etimes":1425,"etitle":"Progress in HD-Zip Transcription Factors of Plant","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"HD-Zip转录因子; 蛋白质结构; 分类; 功能","netpublicdate":"2009-10-29 09:54:57","pdfile1":"Upload/volpdf/20141029-2009040010 514.pdf","seqno":"11","startpage":514,"status":"1","times":3830,"title":"植物HD-Zip转录因子研究进展","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"浙江大学生物技术研究所, 杭州310029; 1<\/SUP><\/B>浙江大学生命科学学院, 杭州310058","aop":"","author":"赫荣琳 樊荣辉 卢建平1<\/SUP> 林福呈* 刘小红","cabstract":"钙离子是非常重要的第二信使，钙离子信号途径几乎参与到细胞生长发育过程中的各个过程。本研究中主要通过同源置换的基因敲除方法对稻瘟病菌中依赖钙离子/钙调蛋白的蛋白激酶激酶2(MoCMKK2<\/I>)基因的功能进行了分析。结果发现，MoCMKK2<\/I>基因缺失突变体的产孢量显著上升；而在致病性、菌丝生长速率、饥饿条件下的生长、分生孢子萌发率和附着孢形成率等方面跟野生型没有差别。上述结果显示，该基因是一个影响产孢量的负调控基因。这一发现为后续稻瘟菌钙离子信号途径相关基因的研究打下了基础。
","caddress":"Tel: 0571-86971185, E-mail: fuchenglin@zju.edu.cn","cdoi":"32200.14.cjcb.2009.04.0011","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0011","eabstract":"Calicium is an important intracellular second messenger and the Ca2+<\/sup>-signal transduction pathway is involved nearly every aspects of the cellular signal transduction network. In this study， the function of a calcium/calmodulin-dependent protein kinase kinase 2 (MoCMKK2) gene in the development of the rice blast fungus Magnaporthe oryzae was studied with a targeted gene disruption method. The sporulation of the null mutant was dramatically increased; however， the pathogenicity， mycelial growth rates， mycelial growth under starvation， conidial germination， appressorium formation and mating test of the MoCMKK2 null mutant were comparable with those of the wild-type strain. These data suggest that the MoCMKK2 gene is a negative factor to regulate sporulation. These findings will further our understanding of the disease infection cycle of Magnaporthe oryzae.","eaffiliation":"Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; 1<\/sup>College of Life Sciences, Zhejiang University, Hangzhou 310058, China","eauthor":"Rong-Lin He, Rong-Hui Fan, Jian-Ping Lu1<\/sup>, Fu-Cheng Lin*<\/sup>, Xiao-Hong Liu ","ecauthor":"Tel: 86-571-86971185, E-mail: fuchenglin@zju.edu.cn ","ekeyword":"Magnaporthe oryzae; MoCMKK2; homologous combination knock-out strategy; sporulation ","endpage":527,"esource":"This work was supported by the National Natural Science Foundation of China (No.0671351) and the Natural Science Foundation of Zhejiang Province (No.Y304211) ","etimes":1523,"etitle":"Functional Analysis of MoCMKK2 Gene in Magnaporthe oryzae","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"稻瘟病菌; MoCMKK2<\/I>; 同源重组基因敲除策略; 产孢","netpublicdate":"2009-10-29 09:47:56","pdfile1":"Upload/volpdf/20141029-2009040011.pdf","seqno":"12","startpage":521,"status":"1","times":3265,"title":"稻瘟菌MoCMKK2<\/I>基因的功能分析","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"东南大学基础医学院病理与病理生理学系, 南京210009","aop":"","author":"梁 伟 郑 杰*","cabstract":"探讨1α，25二羟维生素D3<\/SUB>[1，25(OH)2<\/SUB>D3<\/SUB>]联合曲古菌素A(trichostatin A， TSA)或金雀异黄素对维生素D不敏感前列腺癌PC-3和DU-145细胞生长的影响及其作用机制。MTT和流式细胞术检测显示1，25(OH2<\/SUB>D3<\/SUB>与TSA联合后对PC-3细胞的生长抑制率以及1，25(OH)2<\/SUB>D3<\/SUB>与金雀异黄素联合后对DU-145细胞的生长抑制率均高于单独用1，25(OH)2<\/SUB>D3<\/SUB>。1，25(OH)2<\/SUB>D3<\/SUB>与TSA联合对PC-3细胞的细胞周期阻滞效果优于单独用1，25(OH)2<\/SUB>D3<\/SUB>， 而对DU-145细胞不如单独用1，25(OH)2<\/SUB>D3<\/SUB>。RT-PCR结果显示1，25(OH)2<\/SUB>D3<\/SUB>与TSA联合用药后， PC-3细胞p21cip1<\/SUP><\/I>mRNA表达水平比各单独用药组高， 而DU-145细胞未见明显变化。PC-3细胞中SMRT<\/I> mRNA表达水平高于DU-145细胞， 而DU-145细胞中CYP24<\/I> mRNA的表达水平高于PC-3细胞， TSA和金雀异黄素可分别抑制SMRT<\/I>和CYP24<\/I>的表达。另外ELISA结果显示金雀异黄素明显下调DU-145细胞中的CYP24表达水平。这些研究结果表明PC-3和DU-145细胞对维生素D不敏感的机制不同， TSA可增强1，25(OH)2<\/SUB>D3<\/SUB>对PC-3细胞的生长抑制作用， 而金雀异黄素则可增强1，25(OH)2<\/SUB>D3<\/SUB>对DU-145细胞的生长抑制作用， 这为临床治疗维生素D不敏感肿瘤提供了新的选择。","caddress":"Tel: 025-83272358, E-mail: jiezheng54@126.com","cdoi":"32200.14.cjcb.2009.04.0012","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0012","eabstract":"To investigate the effect of vitamin D on vitamin D—insensitive prostate cancer cell lines by co-treatment with trichostatin A (TSA) and genistein and the possible mechanisms. MTT assay and flow cytometry showed the growth inhibition rate of combination of 1，25(OH)2<\/sub>D3<\/sub> and TSA in PC-3 cells， and that of combination of 1，25(OH)2<\/sub>D3<\/sub> and genistein in DU-145 cells were both higher than that of using 1，25(OH)2<\/sub>D3<\/sub> alone. Compared to using 1，25(OH)2<\/sub>D3<\/sub> alone， co-treatment with 1，25(OH)2<\/sub>D3<\/sub> and TSA induced G0<\/sub>/G1<\/sub> phase arrest in PC-3 cells. By contrast， this effect of cell cycle arrest was weaker in DU-145 cells. RT-PCR showed 1，25(OH)2<\/sub>D3<\/sub> induced p21cip1<\/sup> mRNA expression in PC-3 cells by co-treatment with TSA， but not in DU-145 cells. Furthermore， the SMRT mRNA expression of PC-3 cells was higher than that of DU-145 cells， and the CYP24 mRNA expressed higher in DU-145 cell than that in PC-3 cells. TSA and genistein inhibited the expression of SMRT and CYP24 mRNA， respectively. In addition， ELISA demonstrated that genistein down-regulated CYP24 protein levels of DU-145 cells obviously. This investigation indicated that the mechanism for 1，25(OH)2<\/sub>D3<\/sub>-insensitivity in prostate cancer cells was different. TSA could enhance the anti-proliferative effect of 1，25(OH)2<\/sub>D3<\/sub> on PC-3 cells， while genistein could make the same effect on DU-145 cells. These findings may provide novel chemotherapeutic regime for 1，25(OH)2<\/sub>D3<\/sub>-insensitive cancers.","eaffiliation":"Department of Pathology and Pathophysiology, School of Basic Medical Science, Southeast University, Nanjing 210009, China","eauthor":"Wei Liang, Jie Zheng*<\/sup> ","ecauthor":"Tel: 86-25-83272358, E-mail: jiezheng54@126.com ","ekeyword":"vitamin D; trichostatin A; genistein; prostate cancer cells ","endpage":534,"esource":"This work was supported by the National Natural Science Foundation of China (No.30540049)","etimes":1430,"etitle":"Trichostatin A and Genistein Enhance the Anti-proliferative Effect of Vitamin D on Prostate Cancer Cells","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"维生素D; 曲古菌素A; 金雀异黄素; 前列腺癌细胞","netpublicdate":"2009-10-29 09:48:04","pdfile1":"Upload/volpdf/20141029-2009040012.pdf","seqno":"13","startpage":528,"status":"1","times":2973,"title":"曲古菌素A或金雀异黄素增强维生素D对前列腺癌细胞PC-3和DU-145的生长抑制作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"山东农业大学植物保护学院环境生物系, 泰安271018","aop":"","author":"陈卫红 李多川","cabstract":"从嗜热子囊菌光孢变种(Thermoascus aurantiacus <\/I>var. levisporus<\/I>)中克隆出β-葡萄糖苷酶基因bgl<\/I>II的全长序列， GenBank注册号为EU263992。将该基因插入巴斯德毕赤酵母Pichia pastoris<\/I>分泌型表达载体pPIC9K中以获得重组质粒， 将重组质粒转导入毕赤酵母中， 大量筛选后获得高效表达β-葡萄糖苷酶的酵母工程菌株。经甲醇诱导6 d， 培养基中β-葡萄糖苷酶的活力可达0.23 U/mg。该酶的最适反应pH为5.0， 最适反应温度为50 ℃。通过DEAE-Sepharose Fast Flow阴离子层析纯化了该重组蛋白质。SDS-PAGE测得该重组蛋白质分子量约为118 kDa。","caddress":"Tel: 0538-8249071, E-mail: lidc20@sdau.edu.cn","cdoi":"32200.14.cjcb.2009.04.0013","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0013","eabstract":"The β-glucosidase bg1II gene was cloned from thermophilic fungus Thermoascus aurantiacus var. levisporus. The accession number in GenBank is EU263992. The gene was ligated with the Pichia pastoris expression vector pPIC9K， resulting in the recombinant plasmid. The recombinant plasmid was transformed into P. pastoris. Highly efficient β-glucosidases from project strain were achieved through massive screening. The project strain was induced with methanol in 6 d. The β-glucosidase was expressed and secreated into the culture with activity of 0.23 U/mg. The β-glucosidase exhibited optimum catalytic activity at 50 ℃ and pH 5.0. The recombinant β-glucosidase was purified by using DEAE-Sepharose Fast Flow chromatography. A molecular mass of the purified enzyme is 118 kDa determined by SDS-PAGE","eaffiliation":"Department of Environmental Biology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China","eauthor":"Wei-Hong Chen, Duo-Chuan Li*<\/sup> ","ecauthor":"Tel: 86-538-8249071, E-mail: lidc20@sdau.edu.cn","ekeyword":"Thermoascus aurantiacus var. levisporus; expression; cellulose; Pichia pastoris; purification ","endpage":540,"esource":"This work was supported by the National High-Tech Research and Development Program of China (863 Program) (No.2008AA05Z403, No.2006AA10Z304)","etimes":1404,"etitle":"Cloning， Expression， Purification and Analysis of Enzymic Properties of Thermostable β-glucosidase from the Thermophilic Fungus","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"嗜热子囊菌光孢变种; 表达; 纤维素酶; 毕赤酵母; 纯化","netpublicdate":"2009-10-29 09:48:10","pdfile1":"Upload/volpdf/20141029-2009040013.pdf","seqno":"14","startpage":535,"status":"1","times":2812,"title":"嗜热真菌热稳定性β-葡萄糖苷酶的克隆表达、纯化及酶学性质","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"漳州卫生职业学院药学系, 漳州 363000; 1<\/SUP>厦门大学生命科学学院, 厦门 361005","aop":"","author":"林美珍 葛丽丽1<\/SUP> 田惠桥1<\/SUP>* ","cabstract":"烟草仅合点端的一个大孢子可发育成雌配子体， 珠孔端的其余3个大孢子都退化。在大孢子母细胞减数I， 多数细胞器聚集在细胞珠孔端， 而细胞合点端的细胞器较少。减数分裂完成后由二分体珠孔端细胞形成的两个大孢子率先同时退化， 而由二分体合点端细胞形成的第三个大孢子退化较晚， 显示与前两个大孢子的退化有时间和空间上的差异。","caddress":"Tel: 0592-2186486; E-mail: hqtian@xmu.edu.cn","cdoi":"32200.14.cjcb.2009.04.0014","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0014","eabstract":"Three megaspores of a tetrad of tobacco will degenerate after meiosis of megaspore mother cell and only chalazal-most one develops. In meiosis I， more organelles of the megaspore mother cell move toward the micropylar end and while fewer toward the chalazal end. After meiosis， a tetrad of four megaspores is formed. Then the two micropylar megaspores， which come from the micropylar cell of the dyad， degenerate first. Afterwards， the third megaspore， which comes from the chalazal cell of the dyad， begins to degenerate， suggesting a temporal and spatial degenerative difference with the former two.","eaffiliation":"Department of Pharmacy, Zhangzhou Medical Vocational College, Zhangzhou 363000, China;1<\/sup>School of Life Sciences, Xiamen University, Xiamen 361005, China","eauthor":"Mei-Zhen Lin, Li-Li Ge1<\/sup>, Hui-Qiao Tian1*<\/sup>","ecauthor":"Tel: 86-592-2186486, E-mail: hqtian@xmu.edu.cn ","ekeyword":"megaspore degeneration; meiosis; tobacco ","endpage":546,"esource":"This work was supported by the National Science Foundation of China (No.30670126) ","etimes":1505,"etitle":"The Ultrastructural Observation on Megaspore Degeneration of Tobacco","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"烟草; 减数分裂; 大孢子退化","netpublicdate":"2009-10-29 09:48:17","pdfile1":"Upload/volpdf/20141029-2009040014.pdf","seqno":"15","startpage":541,"status":"1","times":3031,"title":"烟草大孢子退化的超微结构观察","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"1<\/SUP>广西大学广西亚热带生物资源保护利用重点实验室, 南宁530005; 2<\/SUP>广西作物遗传改良生物技术重点开放实验室, ","aop":"","author":"王日升1,2,3<\/SUP> 李杨瑞2<\/SUP>* 周生茂3<\/SUP> 张 曼3<\/SUP> 方锋学3<\/SUP> 杨丽涛1<\/SUP>","cabstract":"为了研究AgNO3<\/SUB>诱导下纯雌系苦瓜花蕾发育过程中的差异基因表达， 本文运用形态学观察、cDNA-AFLP、RT-PCR和反向Northern杂交等技术在形态学和转录组学水平上对花蕾转雄进行了研究。结果显示: (1)花蕾在AgNO3<\/SUB>处理后第6天出现雄蕊， 第14天形成两性花， 而且两性花中的花粉具有完全生活力， 第22天后恢复全雌花; (2)从128对引物组合中筛选出可获得丰富清晰条带的引物组合36对， 它们从AgNO3<\/SUB>处理的花蕾中分离到11个高度表达的差异片段， 经克隆、测序和在线比对， 有2个未知基因， 9个已知基因， 其中1个与植物性别分化有关的CYP450<\/I>家族基因有高度同源性， 命名为McCYP72A1<\/I>; (3)McCYP72A1<\/I>在AgNO3<\/SUB>处理后2~12 h内的花蕾中表达， 而且在第8 h时表达丰度最高， 但在处理的根、茎和叶以及对照的各个器官中不表达。结果表明， AgNO3<\/SUB>处理可诱导纯雌系苦瓜花蕾中与雄性分化相关基因表达， 进而可能导致处理后14天内雌花发育为两性花。","caddress":"Tel: 0771-3247689, E-mail: liyr@gxaas.net","cdoi":"32200.14.cjcb.2009.04.0015","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0015","eabstract":"To elucidate the differential gene expression during the development of stamens in female flower buds of bitter melon gynoecious line induced with AgNO3<\/sub>， in the present study， it was studied at the levels of morphology and transcription genomics using morphologic observations， cDNA-AFLP， RT-PCR and reverse-Northern hybridization. The results showed as follows: (1) After treated with AgNO3<\/sub>， stamens were generated at the 6th day， and bisexual flowers with viable pollen grains were observed at the 14th day， and gynoecious flowers were recovered since the 22th day. (2) Thirty-six primer combinations could amplify many distinct bands， among them， 11 bands which were strongly expressed only in the AgNO3<\/sub>-treated buds were cloned， sequenced and aligned on line， resulting in discovery of 2 unknown genes and 9 known genes. The sequence showing high similarity with CYP450 gene super family which related to plant sex differentiation was named as McCYP72A1. (3) McCYP72A1 was expressed in the flower buds during the period of 2-12 h after treated with AgNO3<\/sub>， and expressed most strongly at 8 h， but not expressed in the roots， stems， leaves of treatment and the mixed samples of buds， roots， stems， leaves of the control. It suggested that genes related to male differentiation in the flower buds of bitter melon gynoecious line were expressed after treated with AgNO3<\/sub>， which might make female flowers develop into bisexual flowers within 14 days.
","eaffiliation":"1<\/sup>Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530005, China; 2<\/sup>Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning 5","eauthor":"Ri-Sheng Wang 1,2,3<\/sup>, Yang-Rui Li3*<\/sup>, Sheng-Mao Zhou 2<\/sup>, Man Zhang 2<\/sup>, Feng-Xue Fang 2<\/sup>, Li-Tao Yang 1<\/sup>","ecauthor":" Tel: 86-771-3247689, E-mail: liyr@gxaas.net","ekeyword":"bitter melon; gynoecious line; male-induced treatment; cDNA-AFLP; differential gene expression ","endpage":552,"esource":"This work was supported by the National Key Technology R&D Pillar Program (No.2007BAD68B03), National Department Public Benefit Research Foundation (No.nyhyzx07-007-1), Guangxi Youth Foundation Program (No.0991077), Guangxi Natural Science Foundation Prog","etimes":1482,"etitle":"Stamen Development and Gene Expression of Flower of Male-induced Bitter Melon Gynoecious Line","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"苦瓜; 纯雌系; 诱雄处理; cDNA-AFLP; 差异基因表达","netpublicdate":"2009-10-29 09:48:26","pdfile1":"Upload/volpdf/20141029-2009040015 547.pdf","seqno":"16","startpage":547,"status":"1","times":3707,"title":"纯雌系苦瓜花经AgNO3<\/SUB>诱雄后的雄蕊发育和基因表达","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"重庆医科大学公共卫生学院生殖生物学研究室, 重庆400016","aop":"","author":"李红梅 刘学庆 余秋波 董艳玲 丁裕斌 王应雄 何俊琳*","cabstract":"采用二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠早期胚胎绒毛和子宫蜕膜组织细胞的蛋白质组。结果发现一个等电点约6.6、分子量约38 kDa的蛋白质点在蜕膜组织中表达明显下调，经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint， PMF)和数据库搜索鉴定此蛋白质点为膜联蛋白-A1(ANXA1)。进一步采用RT-PCR、Western印迹和免疫组织化学技术分析了ANXA1在胚胎绒毛和子宫蜕膜组织细胞中的表达情况， 证实了ANXA1<\/I> mRNA水平及其蛋白质在蜕膜组织细胞中呈现低水平表达， 实验结果提示ANXA1可能在滋养细胞浸润和胎盘形成过程中发挥重要作用。","caddress":"Tel: 023-68485001, Fax:023-68485008, E-mail: hejunlin_11@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0016","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0016","eabstract":"Differential expression of ANXA1 in villus and deciduas from the first trimester pregnancy was investigated by two-dimensional gel electrophoresis and image analysia. The result showed the expression of a protein spot (isoelectric point is about 6.6， molecular weight is about 38 kDa) decreased significantly in deciduas. This protein spot was identified as ANXA1 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database searching. Low level expression of ANXA1 was further comfirmed in deciduas by RT-PCR， Western blotting and immunohistochemistry. The observation suggested that ANXA1 might play an important role in regulation the invasion of trophoblast and placentation during the early pregnancy.
","eaffiliation":"The Laboratory of Reproductive Biology, The School of Public Health, Chongqing Medical University, Chongqing 400016, China","eauthor":"Hong-Mei Li, Xue-Qin Liu, Qiu-Bo Yu, Yan-Ling Dong, Yu-Bin Ding, Ying-Xiong Wang, Jun-Lin He*<\/sup>","ecauthor":"Tel: 86-23-68485001, Fax: 86-23-68485008, E-mail: hejunlin_11@yahoo.com.cn ","ekeyword":"ANXA1; villus; deciduas; proteomics; implantation ","endpage":558,"esource":"This work was supported by the Key Program of the Natural Science Foundation of Chongqing City (No.2004-47)","etimes":1482,"etitle":"The Expression of ANXA1 between Deciduas and Villus in the First Trimester Pregnancy","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"膜联蛋白-A1; 绒毛; 蜕膜; 蛋白质组学; 胚胎植入","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040016.pdf","seqno":"17","startpage":553,"status":"1","times":3256,"title":"ANXA1在孕早期绒毛和蜕膜组织中的表达作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"同济大学附属同济医院泌尿外科, 上海200065; 1<\/SUP>中国科学院上海生命科学研究院生物化学与细胞生物学研究所, 上海 200031","aop":"","author":"章劲夫 桂亚平 胡佳华1<\/SUP>*","cabstract":"为研究乙醇摄入导致睾丸组织生精细胞慢性损伤的机制， 采用野生型(WT)小鼠和广泛表达人Fas配体(Fas ligand， FasL)的转基因型(TG)小鼠分别用20% (V/V<\/I>)乙醇喂养12周， 应用苏木精-伊红(HE)染色、RT-PCR和Western印迹检测等方法观察了乙醇处理后小鼠生精细胞损伤和FasL表达的变化。HE染色结果显示乙醇喂养后，野生型鼠睾丸组织形态基本正常， 而转基因鼠曲细精管有明显退行性上皮空泡化现象; RT-PCR和Western印迹检测结果显示乙醇喂养12周的WT小鼠和TG小鼠睾丸FasL表达高于对照组(普通饮用水喂养12周)， 两组各自相比有统计学意义(P<\/I><0.05)。研究提示FasL可能参与乙醇摄入引起的睾丸组织生精细胞慢性损伤过程， 其作用机制可能是启动Fas/FasL调控生精细胞凋亡。","caddress":"Tel: 021-54921392, Fax: 021-56050502, E-mail: jinfuzhang@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0017","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0017","eabstract":"To explore the mechanisms of chronic damage of testicular germ cells induced by chronic ethanol on the transgenic mice ubiquitously expressing human FasL， both wild type (WT) mice and transgenic (TG) mice were fed with ethanol (20% V/V) for 12 weeks. The histological changes of testicular germ cells were assessed by HE dying， and the Fas ligand (FasL) expression was detected using RT-PCR and Western blot. The result showed that there was a degenerative change in epithelial component of the tubules in TG mice whereas a normal shape in that of WT mice after a chronic ethanol feeding for both， and the FasL was up-regulated in the testis of ethanol-treated TG and WT mice compared with corresponding vehicle-treated control mice (P<0.05) after a 12-week treatment. These evidences suggest that the up-regulation of FasL may be involved in the chronic damage of testicular germ cells induced by chronic ethanol， and Fas/FasL could act as a regulator for the apoptosis of testicular germ cells.","eaffiliation":"Department of Urology, Tongji Hospital of Tongji University, Shanghai 200065, China; 1<\/sup>Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China)","eauthor":"Jin-Fu Zhang, Ya-Ping Gui, Jia-Hua Hu1*<\/sup>","ecauthor":"Tel: 86-21-54921392, Fax: 86-21-56050502, E-mail: jinfuzhang@yahoo.com.cn ","ekeyword":"Fas ligand; ethanol; apoptosis; testes; transgenic mouse ","endpage":562,"esource":"","etimes":1566,"etitle":"Relation of the Chronic Damage of Testicular Germ Cells and Its Fas Ligand Expression Induced by Ethanol","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"Fas 配体; 乙醇; 细胞凋亡; 睾丸; 转基因小鼠","netpublicdate":"","pdfile1":"Upload/volpdf/20141217-2009040017.pdf","seqno":"18","startpage":559,"status":"1","times":2824,"title":"乙醇导致小鼠生精细胞慢性损伤与FasL表达的关系","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"浙江大学医学院附属第一医院传染病诊治国家重点实验室, 杭州310003;1<\/SUP>中国医学科学院北京协和医学院医学生物学研究所, 昆明650118","aop":"","author":"葛长勇 李鸿钧1<\/SUP> 谢天宏1<\/SUP> 张光明1<\/SUP> 易 山1<\/SUP> 孙茂盛1<\/SUP> 吴南屏*","cabstract":"树突状细胞(dendritic cell， DC)在HIV初次感染和潜伏感染中发挥着关键作用。恒河猴的生物学性质与人类非常接近， 所以恒河猴是研究HIV感染的常用动物模型。以恒河猴为实验动物进行DC的研究首先需要对恒河猴DC的生物学性质有初步的了解。本研究从健康恒河猴外周血中分离单个核细胞， 用rhGM-CSF、rhIL-4和rhTNF-α等细胞因子组合体外扩增诱导恒河猴DC， 成功诱导出具有典型形态特征的DC， 所获DC高表达MHC II类分子和CD86共刺激分子， 在混合淋巴细胞反应中能有效刺激T细胞增殖， 培养至第9天表达CD1a的细胞比例为60.5%±2.0%， 表达CD83的比例为43.8%±1.5%， 辣根过氧化物酶(horseradish peroxidase， HRP)内吞实验说明诱导的猴DC在第6天吞噬能力最强。本研究为以恒河猴为实验动物进行DC的研究打下了基础。","caddress":"Tel: 0571-87236580, E-mail: flwnp@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0018","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0018","eabstract":"Dendritic cell (DC) play a crucial role both in the primary HIV infection stage and in the latent HIV infection period. The rhesus macaque， whose biological characteristics are remarkably similar to those of humans， is a good animal model to study the AIDS pathogenesis. The biological characteristics of the rhesus monkey dendritic cells need to be known before the immune function of rhesus monkey dendritic cells in HIV infection will be studied. Rhesus monkey (Macaca mulatta) monocytes were separated from peripheral blood. Monocytes were proliferated and induced to differentiate toward DC by using recombinant human granulocyte- rhGM-CSF， rhIL-4， and impulsed by using inactive SV40 antigen， and promoted for mature DC by using rhTNF-α The morphocytology of DC was observed under ordinary light microscope and scanning electron microscope. The characteristic dendritic morphology was observed on some DC surface. High expression of HLA-DR and CD86 on rhesus monkey DC were mensurated. Rhesus monkey DC derived from inducement in vitro can cause auto T cell proliferation effectively in mixed lymphocyte reaction (MLR). At day 9 of cell culture， flow cytometric analysis showed that cells with CD1a account for 60.5%±2.0%， and cells with CD83 account for 43.8%±1.5%. DC endocytosis of horseradish peroxidase (HRP) showed that the peak value of endocytic capacity of DC lies at the sixth day of cell culture. This study laid the foundation for the research and development of DC by using rhesus monkey as animal model.
","eaffiliation":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China; 1<\/sup>Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking U","eauthor":"Chang-Yong Ge, Hong-Jun Li1<\/sup>, Tian-Hong Xie1<\/sup>, Guang-Ming Zhang1<\/sup>, Shan Yi1<\/sup>, Mao-Sheng Sun, Nan-Ping Wu*<\/sup>","ecauthor":"Tel: 86-571-87236580, E-mail: flwnp@yahoo.com.cn ","ekeyword":"rhesus monkey; dendritic cells; biological characteristics ","endpage":568,"esource":"This work was supported by the National S&T Major Project of China during the 11th Five-Year Plan Period (No.2008ZX10103) and the Opening Foundation of the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospi","etimes":1458,"etitle":"Preliminary Studies on Biological Characteristics of Rhesus Monkey (Macaca mulatta) Dendritic Cells","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"恒河猴; 树突状细胞; 生物学特性","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040018.pdf","seqno":"19","startpage":563,"status":"1","times":2949,"title":"恒河猴树突状细胞生物学特性的初步研究","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"中国医学科学院皮肤病研究所, 南京210042; 1 <\/SUP>南京航空航天大学仿生结构与材料防护研究所, 南京210016","aop":"","author":"弓娟琴 沈丹蓓 曾学思 郭 策1<\/SUP> 戴振东1<\/SUP>*","cabstract":"用苏木素-伊红染色， 在显微镜下观察壁虎脚趾皮肤的组织学结构。对壁虎脚趾刚毛侧皮肤进行真皮、表皮分离， 参照皮肤上皮细胞的培养方法对壁虎表皮进行试验性体外原代培养，
并观察培养细胞的形态及其变化过程。本研究旨在探讨体外培养壁虎脚底刚毛细胞的可行性， 为研制基于人工培养的刚毛阵列作为仿壁虎机器人粘附脚垫提供依据。组织学观察发现， 壁虎脚底表皮细胞主要由多层索形细胞和单层圆形细胞组成， 在表层细胞的远端可见整齐排列的刚毛。用胰蛋白酶分离后可将表皮细胞分为两大类， 分别为胰蛋白酶敏感细胞和胰蛋白酶不敏感细胞， 对胰蛋白酶不敏感的三种细胞胞体较大， 其中表面有突起的细胞可能是成熟的刚毛细胞， 而胞壁光滑有折光的卵圆形细胞很可能是即将脱落或凋亡的刚毛细胞; 对胰蛋白酶敏感的圆形贴壁细胞有可能是尚未成熟或正在发育中的刚毛细胞。体外培养结果发现， 壁虎脚的表皮细胞包含多种细胞成分， 除与人类表皮细胞相似的角质形成细胞样细胞和色素样细胞外， 还发现了有别于人类表皮细胞的三类细胞， 我们暂且称为未确定细胞1、2、3。未确定细胞1与人类的神经细胞极为相似， 推测这种细胞很可能是感应或调节壁虎脚底刚毛细胞运动的神经细胞; 未确定细胞2和3可能均为壁虎脚底的刚毛细，
形态不同可能与刚毛细胞所处的不同生长发育阶段有关， 未确定细胞2可能是处于生长期的刚毛细胞， 而未确定细胞3则可能是成熟的刚毛细胞。","caddress":"Tel: 025-84892581, E-mail: zddai@nuaa.edu.cn","cdoi":"32200.14.cjcb.2009.04.0019","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0019","eabstract":"Histological structures of gecko toe， dyed by hematoxylin-eosin， were observed by an optical microscopy. The hypodermis and epidermis were separated from seta side of the gecko toe， primary culture of the epidermis was carried out in vitro according to the procedure of human epithelium culture and the morphology of cells and their changes were observed under microscopy. The research aims to culture the seta cell in vitro and to explore a new method for manufacturing artificial seta on adhesive pads for gecko-like robot. The histological results showed that epidermal cell of the gecko toe were composed of multilayer drum-like cell and single layer round cell， the seta were arrayed out from the distal end of the round cell. After separation by trypsogen， the epidermal cell could be classified into two cells. Three cells were not sensitive to the trypsogen. One of them with protuberant on the surface was possible the maturated seta cell， and the other with refringent orbicular-ovate was much possible the seta cell of shedding or apoptosis. The culture results showed that there were five cells in the epidermal layer of gecko foot; one cell was similar to the human malpighian cell and another similar to pigment cell. Other three cells could not be found in human epidermis， we called them as un-defined cell 1， 2 and 3. The un-defined cell 1 looked like human nerve cell， it was supposed to be sensory cell which detected the interaction force between toes and ground. The un-defined cell 2 and 3 were possible seta cells of gecko foot， and the differences in morphology may be corresponding to the cell developmental stage. The un-defined cell 2 was possible setae cell under growth period. The un-defined cell 3 was possible maturated seta cell.","eaffiliation":"Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210042, China; 1<\/sup>Institute of Bio-inspired Structure and Surface Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China","eauthor":"Juan-Qin Gong, Dan-Pei Sheng, Xue-Si Zeng, Ce Guo1<\/sup>, Zhen-Dong Dai1*<\/sup>","ecauthor":"Tel: 86-25-84892581, E-mail: zddai@nuaa.edu.cn","ekeyword":"G. gecko; seta; histological structure; cell culture ","endpage":574,"esource":"The work were supported by the National Natural Science Foundation of China (No.60535020 and No.30770285) and the National High-Tech Research and Development Program of China (863 Program) (No.2007AA04Z201)","etimes":1511,"etitle":"Histological Structure and Culture of Seta in Vitro of Gecko Foot","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"大壁虎; 刚毛; 组织学结构; 细胞培养","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040019.pdf","seqno":"20","startpage":569,"status":"1","times":3575,"title":"壁虎脚底刚毛的生物组织结构及其体外培养","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"温州大学生命与环境科学学院, 温州325003","aop":"","author":"钱晓薇* 林国栋 罗蔚华 李玲玲 董美洲","cabstract":"本实验以清洁级ICR雄性小白鼠为实验动物， 研究了不同浓度缢蛏对60<\/SUP>Co γ射线辐照致雄性小白鼠遗传损伤的修复作用。6~7周龄小白鼠辐射后分别灌胃100 mg/(kg⋅d)、200 mg/(kg⋅d)、400 mg/(kg⋅d)剂量的缢蛏10天。采用小白鼠骨髓嗜多染红细胞(polychromaticerythrocytes， PCE)微核实验、小白鼠精子畸形实验等方法， 测定小白鼠胸腺指数及脾指数、骨髓有核细胞数及PCE微核率、小白鼠精子畸形率等指标。实验结果表明: 3个剂量缢蛏组均能使由60<\/SUP>Co γ射线辐照引起的小白鼠胸腺指数及脾指数、骨髓有核细胞数下降得到显著回升(P<\/I><0.001); 3个剂量缢蛏组均能使由60<\/SUP>Co γ射线辐照引起的小白鼠PCE微核率及精子畸形率升高显著下降(P<\/I><0.001)。因此， 实验剂量的缢蛏对60<\/SUP>Co γ射线辐照引起的雄性小白鼠的遗传损伤具有明显的修复作用。","caddress":"Tel: 0577-88371046, E-mail: qianxiaowei@126.com","cdoi":"32200.14.cjcb.2009.04.0020","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.04.0020","eabstract":"We studied the repairing effect of Sinonovacula constricta on genetic injury of ICR male mice induced by 60Co γ-rays. Mice with 6-7 week age were irrigated respectively by 100 mg/(kg⋅d)， 200 mg/(kg⋅d)， and 400 mg/(kg⋅d) Sinonovacula constricta for 10 d. PCE micronucleus test and sperm deformity test were used to measure the index of spleens and chest glands， the number of karyote and the frequency of PCE micronucleus， and the frequency of sperm deformity. The result showed that the 3 different concentrations of Sinonovacula constricta made increase significantly the index of spleens and chest glands， the number of karyote that had been decreased by 60<\/sup>Co γ-rays (P<0.001). The three different concentrations of Sinonovacula constricta made decrease significantly the frequency of PCE micronucleus and sperm deformity decreased by 60<\/sup>Co γ-rays (P<0.001). The conclusion is that the test concentrations of Sinonovacula constricta have obvious repairing effect on the genetic injury of male mice radiated by 60<\/sup>Co γ-rays.","eaffiliation":"School of Life and Environment Science, Wenzhou University, Wenzhou 325003, China","eauthor":"Xiao-Wei Qian*<\/sup>, Guo-Dong Lin, Wei-Hua Luo, Ling-Ling Li, Mei-Zhou Dong ","ecauthor":"Tel: 86-577-88371046, E-mail: qianxiaowei@126.com ","ekeyword":"Sinonovacula constricta; 60<\/sup>Co γ-rays; the genetic injury; repairing ","endpage":579,"esource":"This work was supported by the Foundation of Yueqing Technology Bureau (No.2004R024)","etimes":1474,"etitle":"The Repairing of Sinonovacula constricta on Mice Irradiated by 60<\/sup>Co γ-Rays","etype":"RESEARCH PAPERS","etypeid":7,"fundproject":"","keyword":"缢蛏; 60<\/SUP>Co γ射线; 遗传损伤; 修复作用","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040020 575.pdf","seqno":"21","startpage":575,"status":"1","times":3286,"title":"缢蛏对60<\/SUP>Co γ射线辐照致小白鼠遗传损伤的修复作用","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"1<\/SUP>重庆医科大学超声影像学研究所, 2<\/SUP>重庆医科大学附属第二医院心血管内科, 3<\/SUP>重庆医科大学附属第二医院老年科, 重庆400010","aop":"","author":"钟世根1<\/SUP> 王志刚1<\/SUP> 凌智瑜2<\/SUP>* 殷跃辉2<\/SUP> 李 巧1<\/SUP> 骆 杰1<\/SUP> 李兴升3<\/SUP>","cabstract":"探讨一种可靠、纯度高的犬骨髓间充质干细胞(BMMSCs)体外提取分离、纯化、培养和鉴定的方法。采用骨髓穿刺、Percoll密度梯度离心法分离出犬骨髓单个核细胞， 结合贴壁培养法获得纯度高的犬BMMSCs， 应用细胞免疫化学和流式细胞仪对犬BMMSCs表面标记蛋白进行细胞鉴定和周期测定。结果显示分离的细胞CD29、CD44均呈阳性表达， CD31、CD34、CD45和vWF的表达为阴性; 88.88%的细胞处在G0<\/SUB>/G1<\/SUB>期; 由此证明所分离的细胞为犬BMMSCs， 2周内经3代培养即可扩增到107<\/SUP>细胞数量级， 纯度可达到99%以上， 为干细胞移植治疗缺血性心脏病提供充足的细胞来源。","caddress":"Tel: 023-63719612, E-mail: lingzhiyu1977@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.04.0021","content1":"","csource":"","ctype":"技术与方法","ctypeid":4,"doi":"10.11844/cjcb.2009.04.0021","eabstract":"To explore the approaches of isolation， culture and identify the canine bone marrow mesenchymal stem cells (BMMSCs) in vitro. Bone marrow mononuclear cells were extracted from canine bone marrow and purified via a density gradient centrifugation on Percoll (density 1.073g/ml)， then proliferated in vitro. Cellular immunohistochemistry study and flow cytometry detection were performed identify the cells and determine the cycle for surface marker protein of canine BMMSCs. Immunohistochemistry detection showed the expression of CD29 and CD44 was positive， and the expression of CD31， CD34， CD45 and vWF was negative. 88.88% cells were in the G0<\/sub>/G1<\/sub> period. Which demonstrates that the separated cells were BMMSCs. The cultured stem cells can be expanded to 107<\/sup> orders of magnitude in 2 weeks， and the purity of more than 99% can be achieved. This may provide sufficient sources of cells for stem cell transplantation in the treatment of ischemic heart disease.","eaffiliation":"1<\/sup>Institute of Ultrasound Imaging of Chongqing Medical University; 2<\/sup>Department of Cardiology, 3Department of Geriatrics of the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China","eauthor":"Shi-Gen Zhong1<\/sup>, Zhi-Gang Wang1<\/sup>, Zhi-Yu Ling2*<\/sup>, Yue-Hui Yin2<\/sup>, Qiao Li1<\/sup>, Jie Luo1<\/sup>, Xing-Sheng Li3<\/sup> ","ecauthor":"Tel: 86-23-63719612, E-mail: lingzhiyu1977@yahoo.com.cn ","ekeyword":"canine; marrow mononuclear cell; mesenchymal stem cell; cell culture technique ","endpage":584,"esource":"This work was support by the National Natural Science Foundation of China (No.30700166, No.30800271) ","etimes":1421,"etitle":"Isolation， Cultivation and Identification of Canine Bone Marrow Mesenchymal Stem Cells in Vitro","etype":"TECHNIQUES AND METHODS","etypeid":8,"fundproject":"","keyword":"犬; 骨髓单个核细胞; 间充质干细胞; 细胞培养技术","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040021.pdf","seqno":"22","startpage":580,"status":"1","times":3372,"title":"犬骨髓间充质干细胞体外分离、培养及鉴定方法","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"石河子大学动物科技学院新疆地方与民族高发病省部共建教育部重点实验室, 石河子832003; 1<\/SUP>石河子大学生命科学学院, 石河子832003","aop":"","author":"任 艳 陈创夫* 乔 军 王 勇1<\/SUP> 郭 乾 张 辉 王鹏雁","cabstract":"建立牛胚胎滋养层细胞的体外分离、培养方法。取怀孕牛45~60天的完整子宫， 无菌条件下收集胚胎子叶， 采用胶原酶消化方法分离细胞。将细胞随机分为两组， 一组用差异贴壁法纯化滋养层细胞， 另一组未纯化则用含15%胎牛血清和滋养层细胞生长添加剂(trophoblast growth supplement， TGS)的DMEM培养基培养细胞。采用台盼蓝和Hoechst 33342细胞染色液对细胞活力及形态学进行分析， 采用免疫细胞化学法进行细胞纯度鉴定。结果显示， 本实验分离的细胞经台盼蓝染色后记录细胞存活率大于90%， 核染可见典型双核特征。纯化组细胞经差异贴壁法纯化后双核滋养层巨细胞(binucleate trophoblast giant cells， TGC)纯度可达95%， 未纯化组细胞中TGC只占45%~50%左右。抗细胞角蛋白抗体检测呈阳性， 抗波形蛋白抗体检测呈阴性。细胞经过4次传代后， 可以存活60天。本试验成功建立了一种快速、简便、能够培养高纯度牛胚胎滋养层细胞的方法， 为进一步研究滋养层细胞与相关病原侵入机制打下了基础。","caddress":"Tel: 0993-2058002, E-mail: ccf-xb@163.com","cdoi":"32200.14.cjcb.2009.04.0022","content1":"","csource":"","ctype":"技术与方法","ctypeid":4,"doi":"10.11844/cjcb.2009.04.0022","eabstract":"To establish a convenient method of purification and culture bovine trophoblast cells. Healthy placental tissues between 45 and 60 days of gestation were obtained and digested by collagen I， and then the cells were randomly divided into two groups. The cells from one group were purified by different speeding adherence. Finally， the cells were cultured in DMEM medium including 15% FCS and 5% trophoblast grouth supplement (TGS). The cellular morphology and vitality were observed by inverted phase contrast microscope and trypan blue staining. The nucleus were observed by Hoechst 33342. Immunocytochemistry was used to examine the expression of cytokeratin and vimentin. The results showed that the method of purification had significant effect. The livability was above 90% and typical two nucleus were stained blue. The isolated binucleate trophoblast giant cell (TGC) were positive for cytokeratin and negative for vimentin throughout the duration of the experiment. The purified cells contained 95% TGC and unpurified cells contained only 45%-50% TGC. Cells could passage four generations and survived for sixty days. We had developed an efficient culture system for differentiated TGC enabling future research in this field.
","eaffiliation":"Laboratory of Xinjiang Endemic and Ethnic Disease, College of Animal Science & Technology, Shihezi University, Shihezi 832003, China; 1<\/sup>College of Life Science, Shihezi University, Shihezi 832003, China","eauthor":"Yan Ren, Chuang-Fu Chen*<\/sup>, Jun Qiao, Yong Wang1<\/sup>, Qian Guo, Hui Zhang, Peng-Yan Wang ","ecauthor":"Tel: 86-993-2058002, E-mail: ccf-xb@163.com ","ekeyword":" cell culture; bovine; trophoblast giant cell ","endpage":588,"esource":"This work was supported by International S&T Cooperation Program of China (No.2006DFA33740) ","etimes":1462,"etitle":"In Vitro Culture of Trophoblast Giant Cells from Bovine Placentomes","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"细胞培养; 牛; 滋养层巨细胞","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040022.pdf","seqno":"23","startpage":585,"status":"1","times":3125,"title":"牛胚胎滋养层巨细胞的体外分离培养","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-08-15 00:00:00","affiliation":"上海理工大学生物系统热科学研究所, 上海200093","aop":"","author":"周新丽* 刘建峰 袁 骥 周国燕","cabstract":"冷冻干燥法是保存红细胞的新方法， 具有常温下长期保存、便于运输等优势。目前红细胞的冻干回收率较低， 研究表明将海藻糖载入细胞内可以提高细胞冻干的回收率。本文首先采用电渗透法将海藻糖载入红细胞， 细胞内海藻糖浓度达到(63.68±2.14) mmol/L。然后将载入海藻糖的红细胞冻干、复水， 冻干复水后红细胞平均数目回收率达到(65.9±2.3)%， 扫描电镜观察显示复水后的红细胞保持了完整的细胞形态。这些数据表明利用电渗透法载入海藻糖后将红细胞冻干， 可以提高红细胞冻干后的回收率。","caddress":"Tel: 021-55271167, E-mail: zjulily@163.com","cdoi":"32200.14.cjcb.2009.04.0023","content1":"","csource":"","ctype":"技术与方法","ctypeid":4,"doi":"10.11844/cjcb.2009.04.0023","eabstract":"Freeze-drying is an ideal alternative for preservation of red blood cells， due to its advantages of room temperature storage， lower weight for transportation. However， the recovery rate of freeze-dried red blood cells is low until now. It has been reported that intracellular trehalose is beneficial for freeze-drying of cells. In this investigation， trehalose was loaded into red blood cells by electropermeabilization first， intracellular trehalose concentration reached (63.68±2.14) mmol/L. Then trehalose-loaded red blood cells were freeze-dried and rehydrated， the recovery rate of red blood cells reached (65.9±2.3)%. Scanning electron microscopy showed the morphology of freeze-dried and rehydrated red blood cells were intact and similar to the fresh ones. These results indicated loading trehalose by electropermeabilization before freeze-drying could increase the recovery rate of freeze-dried red blood cells.","eaffiliation":"Institute of Biothermal Science, Shanghai University of Science and Technology, Shanghai 200093, China","eauthor":"Xin-Li Zhou*<\/sup>, Jian-Feng Liu, Ji Yuan, Guo-Yan Zhou ","ecauthor":"Tel: 86-021-55271167, E-mail: zjulily@163.com ","ekeyword":"electropermeabilization; red blood cell; freeze-drying; trehalose ","endpage":593,"esource":"This work was supported by the Chenguang Plan of Shanghai (No.2008CG54)","etimes":1372,"etitle":"Loading Trehalose by Electropermeabilization Increased the Survival of Freeze-dried Red Blood Cells","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"电渗透; 红细胞; 冷冻干燥; 海藻糖","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040023.pdf","seqno":"24","startpage":589,"status":"1","times":2948,"title":"电渗透法载入海藻糖可以提高红细胞冻干效果","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"","affiliation":"","aop":"","author":"徐兰 刘敏英","cabstract":"中科院上海生命科学研究院生化细胞所干细胞技术平台","caddress":"","cdoi":"32200.14.cjcb.2009.04.0024","content1":"","csource":"","ctype":"创刊四十周年纪念专栏","ctypeid":7,"doi":"10.11844/cjcb.2009.04.0024","eabstract":"","eaffiliation":"","eauthor":"","ecauthor":"","ekeyword":"","endpage":594,"esource":"","etimes":12,"etitle":"","etype":"","etypeid":0,"fundproject":"","keyword":"","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-2009040024.pdf","seqno":"2033","startpage":594,"status":"1","times":1553,"title":"胚胎干细胞培养中的饲养层制备","uploader":"","volid":39,"volume":"第31卷 第4期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"南京大学模式动物中心, 南京大学医学院, 南京210093","aop":"","author":"徐 娜 李朝军*","cabstract":"心脏瓣膜以及膜性室间隔起源于胚胎的心内膜细胞， 由心内膜细胞发生上皮-间充质转化(epithelial-mesenchymal transformation， EMT)而来。EMT过程的发生需要多种信号通路相互协调完成， 心脏房室管(atrioventricular canal， AVC)以及流出道(outflow tract，
OFT)处的心内膜细胞在受到邻近的心肌细胞分泌的诱导因子(如TGF-β、BMP等)的影响下， 起始EMT的发生。研究证实， VEGF、Notch、Wnt以及一些转录因子NFATc等都参与调控了EMT过程。这些基因的异常表达都有可能引发EMT过程发生异常， 导致先天性心脏缺陷的产生。目前国内外关于EMT的研究较为广泛， 但是其复杂的信号调控机制还并不是十分清楚， 因而， 搞清楚调控EMT过程的信号通路有着极其重要的生理和病理学意义。本文就心脏瓣膜发育早期的EMT过程中已知的主要信号通路进行了综述。","caddress":"Tel/Fax: 025-83596289, E-mail: licj@nju.edu.cn","cdoi":"32200.14.cjcb.2009.03.0001","content1":"","csource":"","ctype":"特约综述","ctypeid":1,"doi":"10.11844/cjcb.2009.03.0001","eabstract":"Heart valves and the membranous interventricular septum arise from embryonic endocardial cells by epithelial-mesenchymal transformation (EMT)， which requires the corporation of many signaling pathways. EMT is triggered by inductive stimulus like TGF-β and BMP that are produced by the adjacent myocardium lying at heart atrioventricular canal (AVC) and outflow tract (OFT) regions. Studies have confirmed that VEGF， Notch， Wnt and some transcriptional factors like NFATc participate in the regulation of EMT. The abnormal expression of these genes may cause the abnormal EMT events and lead to congenital cardiac defects. At present， the research about EMT has been broadly conducted， but the complicated signaling mechanisms involved in EMT are still unclear. To clarify the physiological and pathological significance of the signaling pathways involved in EMT， we reviews the main signaling pathways and the molecular and cellular mechanisms in the EMT process during early heart valve development.","eaffiliation":"Model Animal Research Center of Nanjing University, Medical School of Nanjing University, Nanjing 210093, China","eauthor":"Na Xu, Chao-Jun Li*<\/sup>","ecauthor":"Tel/Fax: 86-25-83596289, E-mail: licj@nju.edu.cn ","ekeyword":"heart valve development; EMT; signaling regulation ","endpage":306,"esource":"","etimes":1468,"etitle":"Signaling Regulation of EMT Process in Early Heart Valve Development","etype":"INVITED REVIEWS","etypeid":5,"fundproject":"","keyword":"心脏瓣膜发育; 上皮-间充质转化(EMT); 信号调控","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903001.pdf","seqno":"25","startpage":297,"status":"1","times":3602,"title":"心脏瓣膜发育早期EMT过程的信号调控","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"中国科学技术大学, 安徽省细胞动力学与化学生物学重点实验室, 合肥 230027","aop":"","author":"姜 恺 姚雪彪*","cabstract":"微管正端跟踪蛋白(microtubule plus-end tracking proteins)是一大类在进化上保守的蛋白质。其成员的氨基酸序列， 分子结构以及功能不尽相同， 但它们都有一个共同的特点: 特异地在聚合微管的正端聚集。它们之间通过一些蛋白质结构域相互作用， 这些蛋白质结构域之间适当的相互作用强度保证了其能适应细胞内实时变化的环境。微管正端跟踪蛋白的作用广泛， 它们能够调控微管的动态性， 影响微管与细胞器和信号分子的作用，
以及调节微管骨架网络所受到的力。它们对细胞形态和功能的多个方面都产生重要影响。","caddress":"Tel: 0551-3606304, E-mail: yaoxb@ustc.edu.cn","cdoi":"32200.14.cjcb.2009.03.0002","content1":"","csource":"","ctype":"特约综述","ctypeid":1,"doi":"10.11844/cjcb.2009.03.0002","eabstract":"Microtubule plus-end tracking proteins (+TIPs) are a group of conserved proteins that all concentrate at the polymerizing microtubule plus-end in spite of their differences in molecular architecture and cellular function. Plastic networks are formed through the modest interactions between their functional domains， which make them easily adapt to the dynamic environment. By regulating microtubule dynamics， microtubule attachments with cellular organelles and signaling factors， and exerting forces on microtubule networks， +TIPs play various roles in organizing cell architecture and cellular activities.","eaffiliation":"Anhui Key Lab of Cellular Dynamics & Chemical Biology, University of Science and Technology of China, Hefei 230027, China","eauthor":"Kai Jiang, Xue-Biao Yao*<\/sup> ","ecauthor":"Tel: 86-551-3606304, E-mail: yaoxb@ustc.edu.cn ","ekeyword":"cytoskeleton; microtubules; MAP; microtubule plus-end tracking proteins ","endpage":312,"esource":"","etimes":1424,"etitle":"Progress in Microtubule Plus-end Tracking Proteins","etype":"INVITED REVIEWS","etypeid":5,"fundproject":"","keyword":"细胞骨架; 微管; 微管结合蛋白; 微管正端跟踪蛋白","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903002.pdf","seqno":"26","startpage":307,"status":"1","times":3053,"title":"微管正端跟踪蛋白研究的进展与展望","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"浙江大学细胞生物学研究所, 杭州310058; 1<\/SUP>绍兴市第四医院泌尿外科, 绍兴 312030","aop":"","author":"李 翔 叶利洪1<\/SUP> 李继承*","cabstract":"冬凌草甲素(oridonin， ORI)是一种从冬凌草中提取的贝壳杉烯二萜类活性化合物， 具有消炎、抗菌、抗肿瘤等多种药理学活性。近期研究表明ORI能有效抑制肿瘤细胞增殖， 并通过死亡受体介导、线粒体介导的凋亡特异性信号通路及MAPK途径、PI3K/Akt途径等凋亡非特异性信号通路诱导肿瘤细胞凋亡。也有报道发现ORI可通过自噬帮助部分细胞延缓或阻断凋亡而使细胞存活。","caddress":"Tel: 0571-88208088, Fax: 0571-88208094, E-mail: lijichen@zju.edu.cn","cdoi":"32200.14.cjcb.2009.03.0003","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0003","eabstract":"Oridonin (ORI) is an active natural diterpenoid product isolated from Rabdosia rubescens. It has various pharmacological activities， including anti-inflammation， antibacteria and antitumor. Recent studies have focused on the growth inhibition and apoptosis induced by ORI. The mechanisms of the latter action may involve activation of death receptor-mediated pathways， mitochondria-mediated pathways， MAPK pathways or blockade of PI3K/Akt pathway. Further， the autophagy induced by ORI is reported to facilitate tumor cells to delay or escape partially from apoptosis.","eaffiliation":"Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China, 1Urology Department, Shaoxing Fouth Hospital, Shaoxing 312030, China","eauthor":"Xiang Li, Li-Hong Ye1<\/sup>, Ji-Chen Li*<\/sup> ","ecauthor":"Tel: 86-571-88208088, Fax: 86-571-88208094, E-mail: lijichen@zju.edu.cn","ekeyword":"oridonin; apoptosis; signal transduction ","endpage":318,"esource":"November 5, 2008 Accepted: March 11, 2009 ","etimes":1456,"etitle":"Anti-tumor Activity and Mechanism of Oridonin","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"冬凌草甲素; 凋亡; 信号转导","netpublicdate":"2009-10-30 13:49:26","pdfile1":"Upload/volpdf/20141029-200903003.pdf","seqno":"27","startpage":313,"status":"1","times":3328,"title":"冬凌草甲素抗肿瘤活性及其机制","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"浙江大学医学院生物化学与遗传学系, 杭州 310058","aop":"","author":"辛 爱 唐修文﹡","cabstract":"Nrf2-ARE信号转导通路是当前研究的热点，
其核心分子包括核转录因子红细胞系-2p45(NF-E2)相关因子-2 (nuclear factor erythroid-2p45-related factor 2， Nrf2)、抗氧化反应元件(antioxidant response element， ARE)和Kelch样环氧氯丙烷相关蛋白-1 (Kelch-like ECH-associated protein 1， Keap1)。Nrf2通过与ARE相互作用， 诱导II相解毒酶、抗氧化酶及药物流出泵的表达， 在癌症的化学预防中起重要作用。最新研究表明， Keap1和Nrf2的突变与肿瘤发生有关， 也会导致肿瘤细胞对化疗药物产生耐药性。本文综述了有关Nrf2-ARE信号转导通路与肿瘤发生及耐药性的关系。","caddress":"Tel: 0571-88208266, Fax: 0571-88208266, E-mail: xiuwentang@zju.edu.cn","cdoi":"32200.14.cjcb.2009.03.0004","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0004","eabstract":"The important elements of Nrf2-ARE signal pathway are nuclear factor erythroid-2p45-related factor2 (Nrf2)， antioxidant response element (ARE) and Kelch-like ECH-associated protein1 (Keap1). Nrf2 is a redox-sensitive transcription factor that regulates the expression of electrophile and xenobiotic detoxification enzymes and efflux pumps， which confer cytoprotection against oxidative stress and apoptosis in normal cells. However， treatment of cells with anticancer agent induces expression of Nrf2-regulated genes. Genetic alteration of Keap1 and Nrf2 associated with tumorigenesis and confer resistance to chemotherapy. The development of the field regarding the role of Nrf2-ARE signal pathway in tumorigenesis and drug resistance is discussed.","eaffiliation":"Department of Biochemistry and Genetics, College of Medicine, Zhejiang University, Hangzhou 310058, China","eauthor":"Ai Xin, Xiu-Wen Tang*<\/sup>","ecauthor":"Tel: 86-571-88208266, Fax: 86-571-88208266, E-mail: xiuwentang@zju.edu.cn ","ekeyword":"Nrf2; antioxidant response element; multiple drug resistance ","endpage":324,"esource":"This work was supported by the Joint Fund from the Ministry of Health of China, the Health Bureau of Zhejiang Province (No.419100-W10754), the Science and Technology Department of Zhejiang Province (No.519000-J30840) and the Interdiscipline Research Found","etimes":1529,"etitle":"The Role of Nrf2-ARE Signal Pathway in Tumorigenesis and Drug Resistance","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"Nrf2; 抗氧化反应元件; 多药耐药性","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903004.pdf","seqno":"28","startpage":319,"status":"1","times":4255,"title":"Nrf2-ARE信号通路与肿瘤发生及耐药性的关系","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"复旦大学上海医学院医学神经生物学国家重点实验室, 上海 200032","aop":"","author":"洪小琦 梁 敏 黄 芳*","cabstract":"在生物体内， 蛋白酶体系统起着非常重要的作用， 它调控了细胞的许多生理过程。近几年来， 作为起底物特异性泛素化作用的泛素连接酶(E3连接酶)在神经系统中的研究多有报道。通过对最近神经系统中E3连接酶研究的回顾， 综述了其在神经系统的发育、正常功能及神经系统疾病中的研究进展。大量的证据表明， E3连接酶在神经系统中起着极其重要的调控作用， 对其深入研究必将为神经系统疾病的临床治疗提供可靠的科学依据， 并且可能为揭示生命科学的奥秘提供一些思索。","caddress":"Tel: 021-54237296, Fax: 021-64174579, E-mail: huangf@shmu.edu.cn","cdoi":"32200.14.cjcb.2009.03.0005","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0005","eabstract":"The ubiquitin-proteasome pathway is involved in the degradation of proteins and it's regarded as an important mechanism in controlling many physiological events of cells. Recent years， great progresses on ubiquitin liagses， which act as substrate-specific regulator， have been achieved in the nervous system. In this article， we reviewed recent advances of the ubiquitin ligases in neural development， neural function and nervous system diseases. Further work in this area will hold great promise toward the understanding of life science and treatment of a wide range of neurodegenerative diseases.","eaffiliation":"State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China","eauthor":"Xiao-Qi Hong, Min Liang, Fang Huang*<\/sup>","ecauthor":"Tel: 86-21-54237296, Fax: 86-21-64174579, E-mail: huangf@shmu.edu.cn","ekeyword":"ubiquitylation; ubiquitin-protein ligase (E3 ligase); development; nervous system disease; neurodegenerative diseases ","endpage":330,"esource":"This work was supported by the Shanghai Metropolitan Fund for Research and Development (No.07DJ14005) ","etimes":1424,"etitle":"Function of Ubiquitin-protein Ligase in Nervous System","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"泛素化; 泛素连接酶(E3连接酶); 发育; 神经退行性疾病; 神经系统疾病","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903005 325.pdf","seqno":"29","startpage":325,"status":"1","times":3211,"title":"泛素连接酶在神经系统中的作用","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"上海交通大学附属第一人民医院消化疾病研究室, 上海200080; 1<\/SUP>中国海洋大学食品科学与工程学院, 青岛266006","aop":"","author":"陶凯忠 唐庆娟1<\/SUP> 郑 萍*","cabstract":"潘氏细胞是位于肠隐窝底部的浆液性腺上皮细胞， 是构成小肠黏膜屏障的重要细胞成分。潘氏细胞来源于小肠干细胞， 后者可能是位于潘氏细胞上方的标记保留细胞， 也可能是位于潘氏细胞之间的肠隐窝基底柱状细胞。潘氏细胞与杯状细胞和肠内分泌细胞同属于小肠上皮的分泌细胞系， 又与杯状细胞有共同的前体——中间型细胞。潘氏细胞的分化经历了从小肠干细胞到分泌系祖细胞再到中间型细胞的过程。决定潘氏细胞分化、迁移和分布的信号通路主要有Wnt信号通路， 其次是Notch信号通路、Hedgehog信号通路、骨形态发生蛋白信号通路等。","caddress":"Tel: 021-63240090-4255, Fax: 021-63241377, E-mail: zhengpingdoctor@126.com","cdoi":"32200.14.cjcb.2009.03.0006","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0006","eabstract":"Paneth cells are serous glandular epithelial cells at the base of small intestinal crypts. They constitute the key cell component in the intestinal mucosal barrier. These cells originate from the intestinal stem cell (ISC). There are two hypotheses about ISC， i.e. the label-retaining cells above and the crypt base columnar cells intercalated between Paneth cells. Paneth cells， together with goblet and enteroendocrine cells， belong to the secretory cell lineage of the small intestinal epithelium. Furthermore， Paneth and goblet cells share the common precursor， intermediate cell. The differentiation of Paneth cells undergoes a process from ISC to the secretory cell lineage progenitor and to intermediate cell， which is regulated predominantly by the Wnt signaling pathway. The Notch， Hedgehog and bone morphogenetic protein signaling pathways also have effects on the development of Paneth cells.
","eaffiliation":"Laboratory of Digestive Disease, First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China;1<\/sup>College of Food Science and Engineering, Ocean University of China, Qingdao 266006, China","eauthor":"Kai-Zhong Tao, Qing-Juan Tang1<\/sup>, Ping Zheng*<\/sup> ","ecauthor":"Tel: 86-21-63240090-4255, Fax: 86-21-63241377, E-mail: zhengpingdoctor@126.com","ekeyword":"Paneth cell; Wnt; Notch; Hedgehog; bone morphogenetic protein ","endpage":338,"esource":"","etimes":1398,"etitle":"Development of Paneth Cell and Regulation of Signaling Pathways","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"潘氏细胞; Wnt; Notch; Hedgehog; 骨形态发生蛋白","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903006.pdf","seqno":"30","startpage":331,"status":"1","times":3766,"title":"潘氏细胞的发生及其信号调控","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"河南农业大学牧医工程学院, 郑州450002","aop":"","author":"于高水 杨玉荣 梁宏德*","cabstract":"Toll样受体(Toll like receptors， TLRs)是近年发现并倍受关注的一种识别受体。人们发现其在先天性和获得性免疫应答过程中起到链接点的作用， 因此， TLRs在免疫学领域内具有重要地位。目前对TLRs的研究涉及到其结构、配体、信号通路以及临床应用等。现就TLRs目前的研究进展作一综述。","caddress":"Tel: 0371-63554600, E-mail: hdliang12@163.com","cdoi":"32200.14.cjcb.2009.03.0007","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0007","eabstract":"Toll-like receptors (TLRs) are one of pathogen recognition receptors which have been discovered and focused in recent years. TLRs play an important role in junction of innate immunity and adaptive immunity. Recent researches of TLRs involve in their structure， ligand， signaling pathways and clinical application. This review will focus on the research progress in TLRs.
","eaffiliation":"College of Animal and Veterinary Engineering, Henan Agricultural University, Zhengzhou 450002, China","eauthor":"Gao-Shui Yu, Yu-Rong Yang, Hong-De Liang*<\/sup> ","ecauthor":"Tel: 86-371-63554600, E-mail: hdliang12@163.com","ekeyword":"Toll-like receptors; immunity; structure; ligands; signaling pathways ","endpage":343,"esource":"This work was supported by the National Natural Science Foundation of China (No.30800812)","etimes":1470,"etitle":"Progress in Toll-like Receptors","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"Toll样受体; 免疫; 结构; 配体; 信号通路","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903007.pdf","seqno":"31","startpage":339,"status":"1","times":2865,"title":"Toll样受体研究进展","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"上海交通大学医学院, 上海市免疫学研究所, 上海200025","aop":"","author":"胡朝英 张冬青*","cabstract":"γδ T细胞根据其T细胞抗原受体(TCR) γ、δ链的不同组合可分为多个亚型，由于其亚型的异质性而呈现了不同的生物学特性。最初人们对γδ T细胞的认识只限于其在固有免疫中的作用，然而自上个世纪八、九十年代，越来越多的研究发现γδ T细胞在适应性免疫中也扮演着重要的角色。近年来，γδ T细胞具有的专职性抗原递呈细胞的特性——不仅大量表达专职性抗原递呈细胞的表型分子，而且能够诱导初始αβ T细胞的增殖、分化，并辅助B细胞的免疫应答——已成为人们研究的热点。此外，γδ T细胞通过分泌IL-17、IFN-γ等细胞因子在感染免疫、自身免疫和肿瘤免疫中的重要作用也再次受到关注。","caddress":"Tel: 021-64453049, Fax: 021-64453049, E-mail: dqzhang1333@yahoo.com.cn","cdoi":"32200.14.cjcb.2009.03.0008","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0008","eabstract":"γδ T cells， in accordance with different combinations of their γ/δ chains， can be figured out into a number of subtypes， and display different biological characteristics because of their heterogeneity. Initially， γδ T cells were recognized their function in innate immunity. However， many research publications in 1980's and 1990's found that γδ T cells also played an important role in adaptive immunity. Recently， γδ T cells with the features of the professional antigen-presenting cells， not only presented a large amount of phenotype molecules， but also were able to induce the differentiation and proliferation of naive αβ T cells and contribute to the responses of B cells， which has become a hot spot in the field. In addition， much more focus or attention is given to the functional role of γδ T cells about their secretion of IL-17， IFN-γ and other inflammation related molecules in infection immunity， autoimmunity and tumor immunity.","eaffiliation":"Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China","eauthor":"Chao-Ying Hu, Dong-Qing Zhang*<\/sup> ","ecauthor":"Tel: 86-21-64453049, Fax: 86-21-64453049, E-mail: dqzhang1333@yahoo.com.cn ","ekeyword":"γδ T cells; innate immunity; adaptive immunity; APC; immunoregulation ","endpage":348,"esource":"This work was supported by the National Natural Science Foundation of China (No.30471593, No.30670939), the Shanghai Leading Academic Discipline Project (T0206), the Key Project of Shanghai Commission of Science and Technology (07JC14033)and the Shanghai ","etimes":1414,"etitle":"The Professional Antigen-presentation Function of γδ T Cells","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"γδ T细胞; 固有免疫; 适应性免疫; APC; 免疫调节","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903008.pdf","seqno":"32","startpage":344,"status":"1","times":3558,"title":"γδ T细胞的专职性抗原递呈作用","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"上海交通大学医学院附属瑞金医院, 上海 200025","aop":"","author":"许春娣* 林 凯 周 同","cabstract":"幽门螺杆菌(Helicobacter pylori<\/I>， H. pylori<\/I>) 作为人类最常见的模式病原菌和致病菌之一， 其与胃黏膜上皮接触及定植后， 可通过一系列复杂的生物学过程引起宿主固有免疫和适应性免疫异常， 并可导致胃黏膜损伤、溃疡形成， 甚或癌前病变; 也可累及胃肠道以外多个脏器病变。炎症免疫反应是H. pylori<\/I>黏膜损伤的重要病理生理机制。在此过程中， 凭借胃黏膜复杂而精细的免疫调节功能， 胃上皮细胞不仅是H. pylori<\/I>接触感染的首道防线或受害者， 更可能作为主动或直接参与者， 在局部防御或免疫损伤的病生理过程中发挥了重要调节作用， 这些与作为模式识别受体的天然免疫分子调控密切相关。针对H. pylori<\/I>感染及损伤机制， 如何继续从细胞和分子水平以及细菌与宿主两方面深入研究， 并在此基础上加强免疫干预以及个体化治疗与群体预防策略， 可能是H. pylori <\/I>基础与临床研究的关键问题。","caddress":"Tel: 021-64370045, E-mail: chundixu@hotmail.com","cdoi":"32200.14.cjcb.2009.03.0009","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0009","eabstract":"Helicobacter pylori (H.pylori) is one of the most common pattern pathogenic bacteria and pathogens in human. Their contact with the gastric epithelium and colonization may cause the host innate and adaptive immune abnormalities through a series of complex biological processes， and thus lead to gastric mucosal damage， ulcer or even precancerous lesions， also involve a number of extra-gastrointestinal diseases. Inflammatory reaction is an important pathophysiological mechanism in mucosal lesion of H. pylori infection. During this process， by virtue of the complex and delicate mucosal immune regulation function， probably， gastric epithelial cell is not only the first line of defense against H. pylori infection， but also an initiative or direct participant. It plays an important role in the local defense or pathophysiological process of immune injury， which is closely related to the molecular control in innate immunity as pattern recognition receptors. With the infection and injury mechanism of H. pylori， how to continue the study both on the cellular and molecular level and bacteria versus host， how to strengthen the immune intervention， individualized treatment and social prevention， may be the key issues in the basic and clinical study.
","eaffiliation":"Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China","eauthor":"Chun-Di Xu*<\/sup> , Kai Lin, Tong Zhou ","ecauthor":"Tel: 86-21-64370045, E-mail: chundixu@hotmail.com","ekeyword":"Helicobacter pylori; pathogenic mechanism; gastric mucosa barrier; gastric epithelial cells; immunoregulation ","endpage":354,"esource":"This work was supported by the National Natural Science Foundation of China (No.30770999) and the Key Program of Shanghai Municipal Science & Technology Commission (No.03JC14041)","etimes":1403,"etitle":"Progress in Immunologic Mechanism of Helicobacter pylori Infection and Gastric Mucosa Lesion","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"幽门螺杆菌; 致病机制; 胃黏膜屏障; 胃上皮细胞; 免疫调节","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903009.pdf","seqno":"33","startpage":349,"status":"1","times":3361,"title":"幽门螺杆菌感染及胃黏膜损伤免疫机制进展","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"西南大学生命科学学院, 重庆市水产科学技术重点实验室, 淡水生物生殖与发育教育部重点实验室, 重庆400715","aop":"","author":"韩 飞 刘智皓 吴风瑞 黄宝锋 张未丽 周 宏 王德寿","cabstract":"在哺乳动物胚胎期， 成熟分裂首先在雌性生殖细胞启动， 雄性生殖细胞则停滞于有丝分裂的G0<\/SUB>/G1<\/SUB>阶段。在小鼠的研究发现， 成熟分裂启动时间很可能决定着生殖细胞的分化方向。另一方面， 达到一定阈值浓度的维甲酸(RA)能够启动生殖细胞从有丝分裂到成熟分裂的过渡。体内RA的水平由其合成和降解速率调节， 而Cyp26是调节体内RA水平最关键的酶。因此， 这种由Cyp26调节的RA水平的变化可能从根本上调控着哺乳动物生殖细胞的分化方向(形成精原细胞或卵原细胞)， 最终影响性别的分化。本文主要综述RA、Cyp26<\/I>基因家族在哺乳动物性腺分化过程中的作用， 并探讨了低等脊椎动物特别是鱼类成熟分裂启动及生殖细胞分化的分子机制。","caddress":"Tel: 023-68254062, Fax: 023-68252365, E-mail: wdeshou@swu.edu.cn","cdoi":"32200.14.cjcb.2009.03.0010","content1":"","csource":"","ctype":"综述","ctypeid":2,"doi":"10.11844/cjcb.2009.03.0010","eabstract":"During mammalian fetal development， meiosis is initiated in female germ cells only， while male germ cells undergoing G0/G1 mitotic cell cycle arrest. Further study reveals that timing of meiotic initiation during embryonic development probably determines the direction of germ cell differentiation in mammals. In addition， the proper concentration of retinoic acid (RA) can initiate the mitosis to meiosis transition in vivo. Homeostasis of the RA level is maintained by regulation of its rate of synthesis (ADHs and RALDHs are the key enzyme for RA synthesis) and by controlling its rate of degradation (Cyp26s are the most effective enzyme for RA degradation)， which decides the direction of germ cell differentiation (spermatogonia or oogonia)， and affects the sex differentiation of mammals ultimately. This review focuses on the physiological roles of RA and Cyp26s during the process of gonadal differentiation in mammalian and trying to explore the molecular mechanism of meiosis and germ cells? differentiation in the lower vertebrate， especially fish.
","eaffiliation":"Key Laboratory of Aquatic Organism Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China","eauthor":"Fei Han, Zhi-Hao Liu, Feng-Rui Wu, Bao-Feng Huang, Wei-Li Zhang, Hong Zhou, De-Shou Wang*<\/sup>","ecauthor":"Tel: 86-23-68254062, Fax: 86-23-68252365, E-mail: wdeshou@swu.edu.cn","ekeyword":"meiosis; retinoic acid; Cyp26 family gene; Stra8; germ cell differentiation ","endpage":360,"esource":"This work was supported by the National High Technology Research and Development Program (863 Program) of China (No.2007AA10Z165) and the National Natural Science Foundation of China (No.30770272) ","etimes":1425,"etitle":"Involvement of Retinoic Acid and Cyp26 Family Genes in Germ Cell Differentiation","etype":"REVIEWS","etypeid":6,"fundproject":"","keyword":"成熟分裂; 维甲酸; Cyp26<\/I>基因家族; Stra8<\/I>; 生殖细胞分化","netpublicdate":"","pdfile1":"Upload/volpdf/20141029-200903010 355.pdf","seqno":"34","startpage":355,"status":"1","times":3513,"title":"维甲酸和Cyp26基因家族与生殖细胞分化","uploader":"","volid":40,"volume":"第31卷 第3期"},{"acceptdate1":"","acceptdate2":"2009-06-15 00:00:00","affiliation":"温州医学院生命科学学院、检验医学院, 温州 325035; 1<\/SUP>温州医学院基础医学院, 温州 325035","aop":"","author":"胡 昕 谭 峰1<\/SUP> 徐 莹 郑 易 吕建新","cabstract":"将人细胞色素c<\/I>氧化酶 I亚型(COX I<\/I>)的功能区域性片段加入线粒体导向序列，构建pEGFP-N1-COX I真核表达载体转染SK-N-SH细胞，探讨COX I<\/I>定向表达对叠氮钠(NaN3<\/SUB>)引起的SK-N-SH细胞线粒体功能障碍的保护作用。结果发现转染24 h后外源COX I<\/I>基因在细胞内表达明显增加，并至少持续到72 h。COX I<\/I>基因功能区的线粒体定向表达不仅能维持线粒体膜电势，而且还能抑制NaN3<\/SUB>引起的细胞增殖毒性作用。结果显示COX I<\/I>功能区域性片段可通过保护线粒体功能、减少氧化应激来减轻NaN3<\/SUB>毒性，从而发挥保护细胞的作用。","caddress":"Tel: 0577-86689805, Fax: 0577-86689800, E-mail: ljx@wzmc.net","cdoi":"32200.14.cjcb.2009.03.0011","content1":"","csource":"","ctype":"研究论文","ctypeid":3,"doi":"10.11844/cjcb.2009.03.0011","eabstract":"To study the protective role of targeted expression of cytochrome c oxidase I (COX I) domain regional fragment in mitochondrial dysfunction and oxidative stress of SK-N-SH cells resulting from sodium azide (NaN3<\/sub>)， a mitochondrial targeting sequencing was inserted into domain regional fragment of COX I and the plasmid pEGFP-N1-COX I was constructed and transfected into the SK-N-SH cells. The studies showed that the expression of COX I markedly increased at the time of 24 h after transfection and persisted up to 72 h and directional expression of COX I domain regional fragment could not only maintain the mitochondrial potential but also inhibit the toxic effect on proliferation of cells induced NaN3<\/sub>. These data indicate that COX I domain regional fragment could prevent the cells from NaN