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A Simple Method for Isolating Highly Purified Mouse Sertoli Cells
Li-Juan Tang, Qing-Zhong Wang1*, Cheng-Shui Xu
College of Life Science, Qufu Normal University, Qufu 273165, China; 1College of Bio-engineering, Weifang University, Weifang 261061, China
Abstract: To isolate and culture the Sertoli cells from 2-7 days pup mouse， the testis cell suspension was prepared by using two types of digestion solutions called Digestion I and Digestion II. Repeated centrifugation and repeated adherence selection were used to remove germ cells. Finally， highly purified mouse Sertoli cells were obtained.
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