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Apoptosis of Human Osteosarcoma MG-63 Cell Induced by Curcumin
Yan-Bin Zheng, Hai-Bo Yang, Lan-Ying Chen 1, Qi-Fu Li*, Zhen-Li Zhao, Yong-Jin Liu
School of Life Sciences, Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005, China; 1Pingdingshan Institute of Technology, Pingdingshan 467044, China
Abstract: The apoptosis effects of curcumin (Cur) on human osteosarcoma MG-63 cells have been studied using the methods of cell culture， cell count， flow cytometry analysis， DNA gel electrophoresis， Hoechst33258 staining， HE staining， electron microscope. The results showed that proliferation of MG-63 cells could be inhibited by 7.5 mg/L Cur， and the inhibitory rate is 63.14%. The results of flow cytometry analysis showed that curcumin could induced the emergence of the phase of apoptosis， and the rate is 21.8%. Agarose gel electrophoresis revealed that cell DNA fragment exhibited characteristic "DNA ladder" Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with curcumin appeared shrinked， cell nucleus concentrated， chromatin agglutination， mitochondria swelling， and apoptosis body forming. This study suggested that curcumin had induced apoptosis of the human osteosarcoma MG-63 cells effectively， and provides important foundation and research proofs to study more about the apoptosis mechanisms of the osteosarcoma cells and the study of disease caused by osteoplast.
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