Immuno-gold Localization of Calmodulin Interaction between Barley and Its Powdery Mildew Pathogen， Blumeria graminis f. sp. hoedei

Abstract: The immuno-gold localization of calmodulin (CaM) in near isogenic line barley， Ingrid (Mlo) and BC Ingrid mlo-3 (mlo-3)， induced by powdery mildew attack， was examined. CaM was detectable in all leaf cells and abundant CaM was found in the actively metabolic cell， such as companion cells. CaM existed in nuclei， cell wall， and chloroplast， especially in nuclei. In healthy leaves， the density of labeling for CaM in different cells in Ingrid and mlo-3 were similar except companion cells， in which CaM was much more in Ingrid than in mlo-3. The CaM was increased after inoculation with powdery mildew in other cells and organelles except that in the mesophyll nuclei. The changes of CaM in companion cells and mesophyll cells were also discussed.

CSTR: 32200.14.cjcb.2004.06.0022