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Effect of Bone Marrow Mesenchymal Stem Cell Transplantation on Myocardium Structure and Function in the Rats
De-Qin Jing*, Jie Tian, Yong-Hong Bai2, Tie-Wei Lv, Wen Zhang, Lei Zhang, Jing Zhu1, Yuan Chen, Yong-Ru Qian
Cardiology Department, Children's Hospital of Chongqing Medical University; 1Paediatric Research Institution, Chongqing Medical University; 2Department of Echocardiography, Children's Hospital of Chongqing Medical University, Chongqi
Abstract: The experiment studied effect of bone marrow mesenchymal stem cell (MSC) transplantation on myocardium structure and function in rats. Fifty-four heart failure rat models were induced by doxorubicin among ninety-six female Wistar rats and divided into three groups randomly. In transplantation group， MSC were injected into left ventricular myocardium; control group received equivalent volume culture medium; heart failure group had no surgery. Left ventricular function was assessed by transthoracic echocardiography (TTE) every two weeks. At eight weeks after transplantation， the hearts were excised for histology analysis to observe the morphology changes of injured myocardium and immunohistochemistry analysis to investigate the expression of sarcomeric myosin heavy chain (MHC) and connexin43 (Cx43) in implanted in MSC. The results showed the survival and expression of MHC and Cx43 in implanted MSC. The swelling of adjacent cardiomyocytes was alleviated. Heart functions were improved significantly at 2 weeks after transplantation. At eight weeks， heart functions were improved more significantly. In conclusion， implanted MSC not only may be survival and differentiated into cardiomyocytes in vivo as well as alleviate the damage of myocardium， but also improve heart function of rats with heart failure.
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