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A Simple Rapid Method for Screening Fast-acting Analogs of Monomeric Insulin
Jiang Li1,2, Da-Fu Cui1*
1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Abstract: Lispro insulin produced by genetic engineering was already used to treat diabetes in the world. How to obtain new fast-acting analogs of monomeric insulin by simple and rapid methods has become a hot spot of research. In this article， We report a new approach for screening new fast-acting analogs of monomeric insulin through enzymatic synthesis of Lispro Insulin by DOI (pork insulin with B23-30 removed) from pork insulin digestion with trypsin and GFFYTKPT synthesized by chemical methods. The results indicated that the Lispro Insulin with a purity of 95% produced by enzymatic synthesis has less tendency of self-association as a monomeric insulin.
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